SK-LU-1 Cells
$540.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Organism | Human |
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Tissue | Lung |
Disease | Adenocarcinoma (grade III) |
Synonyms | SK-Lu-1, SK LU 1, SK-Lu1, SK-LU1, SKLU-1, SKLU1, SKLU01 |
Characteristics
Age | 60 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SK-LU-1 (Cytion catalog number 300335) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 positive |
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Antigen expression | Blood Type O, Rh+, HLA Aw24, Aw32, B27, Bw41 |
Isoenzymes | Me-2, 1, PGM3, 1, PGM1, 2, ES-D, 2, AK-1, 1, GLO-1, 2, G6PD, B |
Tumorigenic | Yes, in immunotolerant rats and nu-nu mice |
Karyotype | The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q,11q), 11q+, t(13,?), 16q+, t(12q, 18q). M10, t(2q,13q), i(15), and ?t(xp,21q) occurred in all S metaphases, and t(1p,?), t(1p,14q), t(16,?), and t(14,21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, x chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation. Phenotype Frequency Product: 0.00003 |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10
D13S317: 10
D16S539: 8
D5S818: 11
D7S820: 9
TH01: 7
TPOX: 8,10
vWA: 16,17
D3S1358: 18
D21S11: 29,30.2
D18S51: 18
Penta E: 5
Penta D: 10,13
D8S1179: 10
FGA: 21,22
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HLA alleles |
A*: 24:02:01
B*: 40:02:01
C*: 02:02:02
DRB1*: 13:01:01
DQA1*: 01:03:01
DQB1*: 06:03:01
DPB1*: 04:02:01
E: 01:01:01
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