UM-HMC-3B cells

Product Overview
Volume: 100 ml
Storage: +2°C to +8°C
Sterility: Sterile-filtered
MEM Non-Essential Amino Acids (100x) is a sterile supplement designed to enhance cell growth and viability in mammalian cell culture systems. The formulation corresponds to a 100x concentrate of the non-essential amino acids found in standard Minimum Essential Medium (MEM), allowing for direct supplementation of basal media with minimal preparation.
Application and Benefits This supplement provides an additional amino acid pool for rapidly proliferating cells or for cell lines that have lost the capacity to synthesize non-essential amino acids de novo. By alleviating the metabolic burden of biosynthesis, it supports improved growth kinetics, prolonged viability, and greater experimental consistency—especially in nutrient-sensitive or high-density cultures.
Composition and Use The solution includes glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline, and L-serine. It is compatible with MEM and most other standard media. To use, dilute 1:100 into the final culture medium. This product is sterile-filtered and ready to use without additional handling steps. For research use only. Not for use in diagnostic or therapeutic procedures. Not for use in humans or animals.

Cytion’s Freeze Medium CM-1 is a state-of-the-art cryopreservation medium designed to ensure the highest level of cell viability and functionality post-thaw. This versatile medium is suitable for a broad spectrum of cell types, including both human and animal cells, making it an essential tool for diverse research applications. Formulated with a meticulously balanced combination of cryoprotectants and essential nutrients, Freeze Medium CM-1 minimizes ice crystal formation and cellular stress during the freezing process, thus preserving cellular integrity.
Key features of Freeze Medium CM-1 include:

Broad Compatibility: Effective for a wide range of cell types, including primary cells, stem cells, and established cell lines.
High Viability: Optimized to maximize post-thaw cell recovery and viability, ensuring reliable experimental outcomes.
Ready-to-Use: Conveniently prepared and sterilized for immediate application, reducing preparation time and risk of contamination.
Enhanced Stability: Maintains consistent performance under standard cryopreservation conditions, ensuring reproducible results.
Long Shelf Life: CM-1 is a serum-containing, ready-to-use cryopreservation medium that can be stored in the refrigerator for up to one year.


Using CM-1 for Freezing Cells

To use CM-1 for freezing both adherent and suspension cells, follow these steps:


For adherent cells, wash and dissociate them from the culture substrate. For suspension cells, proceed directly to the next step.
Count the cells to ensure they are at the proper concentration.
Centrifuge the cells to pellet them, then resuspend in CM-1 freeze medium.
Transfer the resuspended cells into cryovials.
Use a slow-freezing method before transferring the cells to long-term storage.






Method
Description
Steps





❄️
Manual Freezing


A step-by-step method involving gradual temperature reduction to ensure cell viability.


1️⃣ Place cells in freeze medium in a 4°C freezer for 40 minutes.
2️⃣ Transfer to a -80°C freezer for 24 hours.
3️⃣ Store cells in liquid nitrogen for long-term preservation.




❄️
Using Mr. Frosty


A convenient device that allows for controlled freezing rates without electrical power.


1️⃣ Prepare cells in cryovials with freeze medium.
2️⃣ Place cryovials in Mr. Frosty container.
3️⃣ Store at -80°C for 24 hours before transferring to liquid nitrogen.




❄️
Controlled-Rate Freezer


A high-precision freezer by Thermo Fisher or other manufacturers designed for controlled temperature reduction.


1️⃣ Program the device to gradually decrease the temperature.
2️⃣ Place prepared cells in the freezer.
3️⃣ After the freezing cycle, transfer cells to liquid nitrogen.










Store the cryovials at temperatures below -130°C or in liquid nitrogen for long-term preservation.


Ingredients

Contains FBS, DMSO, Glucose, Salts
Buffering capacity: pH = 7.2 to 7.6


Cytion’s Freeze Medium CM-1 offers a reliable solution for cryopreservation, ensuring high cell viability and functionality post-thaw for a wide range of research applications.

Accutase Cell Detachment Solution with EDTA and Phenol Red – 100 ml

Accutase is a ready-to-use, sterile-filtered cell detachment solution designed as a gentle alternative to trypsin/EDTA for dissociating adherent mammalian cells from standard tissue culture plasticware and adhesion-coated surfaces. It combines proteolytic and collagenolytic enzyme activity in a balanced salt solution to deliver effective yet controlled dissociation, preserving cell-surface proteins and supporting high post-passage viability and rapid reattachment.

The Accutase formulation is based on Dulbecco’s phosphate-buffered saline (DPBS) with EDTA and phenol red as a visual pH indicator. The enzymes are of non-mammalian and non-bacterial origin, making Accutase particularly well suited to stem cell research, vaccine workflows, and any application where animal
- or microbially-derived contaminants must be minimised. The solution auto-inhibits at 37 °C, so no neutralising reagent or serum-containing medium is required after detachment – cells can be transferred directly into fresh medium.

Key Features

Ready-to-use 1x sterile-filtered liquid – no dilution or reconstitution required
Combined proteolytic and collagenolytic enzyme activity for gentle dissociation
Each batch standardised to a defined dissociation activity for lot-to-lot consistency
Non-mammalian and non-bacterial enzyme origin
Auto-inhibits at 37 °C – no neutralising solution needed
Formulated in Dulbecco’s PBS with EDTA
Phenol red included as visual pH indicator
pH 6.8 – 7.8


Typical Applications
Accutase gently dissociates a wide variety of adherent and sensitive cell types, including human embryonic stem cells (hESCs), human induced pluripotent stem cells (iPSCs), neural stem cells, primary neurons, and routinely cultured adherent lines such as HeLa, HEK 293, CHO, MDCK, Vero, NIH/3T3, BHK-21 and A549. Typical use cases include:

Routine subculture and passaging of adherent mammalian cells
Gentle single-cell dissociation of hESCs, iPSCs and other sensitive lines
Sample preparation for flow cytometry and FACS analysis
Analysis of cell-surface markers where epitope integrity matters
Cell migration, proliferation and apoptosis assays
Quiescence assays by serum starvation and oncogene transfection studies
Tumor cell and neural crest cell migration assays
Production scale-up in bioreactor workflows

For routine work, apply approximately 10 ml of Accutase per 75 cm2 of culture surface and incubate for 5–10 minutes at room temperature. The optimal incubation time should be determined for each cell line and should not exceed one hour. Prior to addition, rinse the cell layer with a Ca2+/Mg2+-free salt solution such as DPBS without calcium and magnesium to remove residual serum and divalent cations.

Handling & Storage
Store the unopened bottle frozen at -15 °C or below. Thaw either at room temperature or overnight at +2 °C to +8 °C. Do not thaw Accutase in a 37 °C water bath, as elevated temperatures reduce enzyme activity. After thawing, the solution can be stored for up to 2 months at +2 °C to +8 °C; do not store at room temperature. Do not pre-warm the reagent to 37 °C before application – add it directly to washed cells at room temperature. For long-term shelf life, single-use aliquoting is recommended to avoid repeated thaw cycles. Always work under aseptic conditions.

Quality
Manufactured under strict quality standards. Each batch of Accutase is sterile-filtered and tested for sterility, pH, appearance and dissociation activity to ensure consistent, reproducible performance from lot to lot.

Product Specifications



Specification
Detail



Product typeCell detachment / dissociation reagent
FormatSterile-filtered liquid, ready-to-use
Volume100 ml
Working concentration1x (ready-to-use)
Enzyme activityCombined proteolytic and collagenolytic
Enzyme originNon-mammalian and non-bacterial
Buffer systemDulbecco’s PBS with EDTA
pH indicatorPhenol red
pH range6.8 – 7.8
AppearanceClear, pale red to orange solution
Storage temperature-15 °C or below
Stability after thawingUp to 2 months at +2 °C to +8 °C
Recommended use volume~10 ml per 75 cm2 culture surface
Typical incubation time5 – 10 minutes at room temperature
Shipping conditionsFrozen on dry ice
Intended useFor research use and further manufacturing only



Formulation (Composition per Liter)



Component
Concentration (mg/L)




Inorganic Salts

Sodium chloride (NaCl)8000.00
Disodium hydrogen phosphate (Na2HPO4)1150.00
Potassium chloride (KCl)200.00
Potassium dihydrogen phosphate (KH2PO4)200.00

Other Components

EDTA · 4Na (tetrasodium EDTA)220.00
Phenol red3.00
Proprietary enzyme blend (proteolytic and collagenolytic activity)1x



Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Organism	Human
Tissue	Oral cavity, hard palate
Disease	Hard palate mucoepidermoid carcinoma

Organism	Human
Tissue	Salivary gland, anterior buccal gland
Disease	Salivary gland mucoepidermoid carcinoma