RKO-E6 Cells


General information
Description | RKO-E6 cells are a human colorectal carcinoma cell line derived from the RKO cell line through additional mutagenesis. These cells are commonly used in cancer research, particularly focusing on colorectal cancer. The E6 variant of the RKO line offers a distinct profile that is useful for examining the effects of specific genetic manipulations and studying the molecular mechanisms of tumorigenesis and metastasis in colorectal cancer. RKO-E6 cells are characterized by several unique features, including alterations in genes related to cell cycle regulation, apoptosis, and DNA repair pathways. These modifications enhance the cell line's utility for investigating the biological effects of gene silencing or overexpression within a colorectal cancer context. For example, RKO-E6 cells have been employed to study the impact of tumor suppressor genes and oncogenes on cancer cell behavior, including proliferation, invasion, and resistance to chemotherapeutic agents. Furthermore, RKO-E6 cells are useful in studies aimed at understanding the cellular responses to environmental stressors, such as oxidative stress and DNA-damaging agents, which are relevant to the pathogenesis and progression of colorectal cancer. Their robust growth characteristics and genetic stability make them a valuable model for high-throughput screening assays to evaluate the efficacy of new anticancer compounds. In summary, RKO-E6 cells provide a critical model for advancing our knowledge of colorectal cancer biology and for developing and testing novel therapeutic strategies targeted at this prevalent and often deadly disease. |
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Organism | Human |
Tissue | Colon |
Disease | Colon carcinoma |
Synonyms | RKOE6 |
Characteristics
Morphology | Epithelial |
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Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | RKO-E6 (Cytion catalog number 305135) |
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Biosafety level | 2 |
Expression / Mutation
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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