MS751 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

€550.00*

Prices excl. taxes plus shipping costs

Product number: 305115

Safety data sheet

Product sheet

Description	MS751 is a tumorigenic human cervical carcinoma cell line isolated from the uterus of a female patient with epidermoid carcinoma. The cells were originally obtained from a metastatic lymph node, and they form poorly differentiated epidermoid carcinoma (grade III) when xenografted into nude mice. The tumorigenic and metastatic nature of MS751 cells makes them a valuable model for studying the processes involved in cervical cancer metastasis and tumor progression. These cells are particularly useful for investigating epithelial-to-mesenchymal transition (EMT), invasion, and metastasis, especially in relation to poorly differentiated carcinoma. One of the key molecular features of MS751 is the presence of human papillomavirus (HPV) sequences. Originally reported to contain HPV-18, more recent studies have demonstrated that MS751 cells contain partial sequences of HPV-45, particularly from the E6/E7 region, which are expressed as poly(A)+ RNA. The E6 and E7 oncoproteins are well-known for their roles in disrupting the tumor suppressor functions of p53 and Rb, respectively, which promote uncontrolled cell division and contribute to oncogenesis. The presence of these viral sequences makes MS751 highly relevant for studies on HPV-associated cervical cancers, and specifically for investigating how HPV-45 contributes to the malignancy of cervical cells. MS751 cells exhibit epithelial morphology, which is characteristic of many cervical cancer cell lines. They are widely used for research into the molecular mechanisms underlying HPV-mediated carcinogenesis, as well as for drug discovery and therapeutic screening. Given their metastatic origin and the presence of HPV sequences, MS751 provides an essential model for studying the progression of cervical cancer and testing therapeutic strategies aimed at targeting both viral and tumor-related pathways.
Organism	Human
Tissue	Cervix
Disease	Human papillomavirus-related cervical squamous cell carcinoma
Metastatic site	Lymph node
Synonyms	MS-751, MS 751

Age	47 years
Gender	European
Morphology	Epithelial
Growth properties	Adherent

Citation	MS751 (Cytion catalog number 305115)
Biosafety level	1

Antigen expression	Blood Type AB, Rh＋
Tumorigenic	Yes, in nude mice, forms poorly differentiated epidermoid carcinoma (gradeⅢ).
Viruses	HPV18, HPV45

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Medium supplements	Supplement the medium with 10% FBS and 1% NEAA
Passaging solution	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1:2 to 1:4
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Handling of cryopreserved cultures	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

MS751 Cells

General information

Characteristics

Identifiers / Biosafety / Citation

Expression / Mutation

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Quality control / Genetic profile / HLA