SK-MEL-1 Cells

€550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300424

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Material Transfer Agreement

Description	This cell line was established in 1966 by F. Oettgen and associates using cells from the thoracic duct of a patient. Pigment granules relating both to synthesis and to phagocytosis are present. According to our sequencing, WB and PCR results this cell line carries a BRAF V600E mutation. Cells are N-Ras wildtype.
Organism	Human
Tissue	Skin
Disease	Melanoma
Metastatic site	Thoracic lymph duct
Synonyms	SK-Mel-1, SK Mel 1, SK-Mel 1, SK-Mel1, SKMEL-1, SkMEL-1, SKMEL1, SK 1

Age	29 years
Gender	Male
Ethnicity	Caucasian
Morphology	Spherical
Growth properties	Suspension

Citation	SK-MEL-1 (Cytion catalog number 300424)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0068

Antigen expression	Blood Type A, Rh+. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.
Isoenzymes	PGM3, 1, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B,
Tumorigenic	Yes, in nude mice. Forms pigmented malignant melanomas. Also forms tumors in the cheek pouch of cortisone treated hamsters
Products	Melanin
Mutational profile	V600E type BRAF Mutation was determined by DNA based methods (sequencing, RT-PCR) and protein based methods (Western Blot)

Culture Medium	RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 15% heat-inactivated FBS
Dissociation Reagent	Accutase
Subculturing	Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 10⁵ cells/ml and keep the cell concentration within the range of 3 x 10⁵ to 1 x 10⁶ cells/ml for optimal growth.
Seeding density	1 to 2 x 10⁵ cells/mL
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300424-271025	Certificate of Analysis	05. Dec. 2025	300424
300424-812	Certificate of Analysis	23. May. 2025	300424

If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.

For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.

Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement.

SK-MEL-1 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)

Material Transfer Agreement