SK-N-LO Cells

€800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300400

Safety data sheet

Product sheet

Material Transfer Agreement

Description	The SK-N-LO cell line is a human neuroblastoma cell line used in research to study neuroblastoma as well as mechanisms of apoptosis and cancer signaling pathways. It is also classified as a primitive neuroectodermal tumor (PNET) cell line and carries the EWS-FLI1 fusion gene, commonly found in Ewing's sarcoma family tumors (ESFT). This fusion gene results from a chromosomal translocation and plays a key role in the oncogenic behavior of these tumor cells. SK-N-LO cells are particularly sensitive to certain inhibitors targeting oncogenic signaling pathways. For example, the GLI inhibitor GANT61 has been shown to induce caspase-independent apoptosis in SK-N-LO cells. GANT61 disrupts GLI1 and GLI2-mediated transcription in the Hedgehog (Hh) signaling pathway, which is critical for cell survival and proliferation in this cell line. When treated with GANT61, SK-N-LO cells exhibit morphological changes associated with apoptosis, such as chromatin condensation and nuclear fragmentation. Furthermore, GANT61 reduces the expression of proteins like GLI2 and survivin, which are important for cell cycle progression and survival, while increasing the expression of p21, a cyclin-dependent kinase inhibitor. Additionally, SK-N-LO cells have been utilized to study opioid receptor signaling. These cells have been engineered to express the μ-opioid receptor, making them a valuable model for investigating the interaction between opioid-induced analgesia and intracellular signaling pathways. For instance, studies have shown that morphine stimulates Akt phosphorylation in SK-N-LO cells via the PI3Kγ pathway, a process that can be modulated by cAMP signaling. This highlights the versatility of SK-N-LO cells in exploring both cancer biology and neuropharmacology.
Organism	Human
Tissue	Brain
Disease	Primitive Neuroectodermal tumor
Metastatic site	Bone marrow
Synonyms	SK-N-L0, SKN-LO, SKNLO

Age	10 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth properties	Adherent in collagen-coated flasks

Citation	SK-N-LO (Cytion catalog number 300400)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_4569

Karyotype	Phenotype Frequency Product: 0.00005

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	3 to 4 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use 50% basal medium + 40% FBS + 10% DMSO, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.

For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.

Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement.

SK-N-LO Cells

General information

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Regulatory Data

Biomolecular Data

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Material Transfer Agreement