KYSE520 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305449

Fiche signalétique

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Certificat d'analyse (CoA)

Description	The KYSE520 cell line is a human esophageal squamous cell carcinoma (ESCC) model derived from a primary tumor. It is moderately differentiated and has been instrumental in investigating epithelial-mesenchymal plasticity (EMP) in esophageal cancer. KYSE520 cells exhibit heterogeneity, consisting of both epithelial-like (CD44v+) and mesenchymal-like (CD44v−) subpopulations. These two populations are capable of interconversion, reflecting a dynamic EMP process. This property makes KYSE520 an excellent model for studying cancer stem cell traits and chemoresistance mechanisms in ESCC. Genetically, KYSE520 cells display notable epigenetic regulation. The promoter region of the JAM3 gene, a tumor suppressor, is unmethylated in these cells, allowing its expression. JAM3 plays a role in regulating cell proliferation, migration, and invasion through Wnt/β-catenin signaling. The maintenance of JAM3 expression in KYSE520 has been linked to the suppression of aggressive cancer phenotypes. In therapeutic research, KYSE520 cells have been used to explore the role of fibroblast growth factor receptor-like 1 (FGFRL1). Studies have shown that FGFRL1-deficient KYSE520 cells exhibit reduced tumor growth and motility, alongside a decrease in matrix metalloproteinase-1 (MMP-1) and fibroblast growth factor binding protein 1 (FGFBP1) expression. These findings underscore the importance of FGFRL1 in tumorigenesis and suggest potential therapeutic targets. Additionally, the EMP dynamics and associated molecular pathways in KYSE520 cells provide insights into ESCC progression and resistance mechanisms, contributing to the development of targeted treatments.
Organisme	Human
Tissu	Esophagus
Maladie	Squamous cell carcinoma
Synonymes	KYSE 520, KYSE-520, Kyse520, KYSE0520

Âge	58 years
Genre	Female
Origine ethnique	Japanese
Morphologie	Epithelial-like
Propriétés de croissance	Adherent, monolayer

Référence	KYSE520 (Cytion catalog number 305449)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_1355

Oncogènes	TP53, MYC
Profil mutationnel	Mutation: TP53, c.376-2A>T, Splice acceptor mutation

Milieu de culture	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a) + RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a); 1:1 mixture
Suppléments	Supplement the medium with 2% FBS
Réactif de dissociation	Accutase
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densité de semis	0.6 - 1.2 x 10⁴ cells/cm²
Renouvellement des fluides	2 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305449-020125	Certificat d'analyse	05. Mar. 2026	305449
305449-170426	Certificat d'analyse	15. May. 2026	305449

KYSE520 Cells

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Certificat d'analyse (CoA)