KYSE-410 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305122

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Certificat d'analyse (CoA)

Description	KYSE-410 is a human esophageal squamous cell carcinoma (ESCC) cell line that was established from a primary tumor resected from an adult patient. This cell line is part of the KYSE series, which includes multiple ESCC models designed to provide a comprehensive tool for studying the various aspects of esophageal cancer. KYSE-410 cells have a doubling time of 24.2 hours, reflecting a moderate proliferative capacity. They grow as adherent monolayers, a common feature among epithelial-derived cancer cells, and exhibit a relatively uniform morphology under phase-contrast microscopy. At the genetic level, KYSE-410 is particularly notable for its epigenetic alterations. The p16 (INK4a) gene in KYSE-410 shows hypermethylation of the 5' CpG islands, a modification that leads to the silencing of this crucial tumor suppressor gene. This epigenetic change is a significant driver of oncogenesis in many cancers, including ESCC, as it results in the loss of cell cycle regulation and unchecked cell proliferation. Despite this, KYSE-410 retains a wild-type configuration for the p15 (INK4b) gene, highlighting a selective inactivation of p16 that is typical of certain cancer subtypes. The KYSE-410 cell line is tumorigenic, as demonstrated by its ability to induce tumor formation when implanted into athymic nude mice. The histological analysis of these tumors shows features consistent with squamous cell carcinoma, making KYSE-410 a relevant model for in vivo studies. This cell line is highly valuable for research focused on understanding the role of epigenetic modifications in cancer progression, as well as for testing the efficacy of therapies targeting epigenetic regulators, although it is not intended for therapeutic or in vivo applications.
Organisme	Human
Tissu	Esophagus
Maladie	Esophageal squamous cell carcinoma
Synonymes	KYSE 410, KYSE410, Kyse410, KYSE0410

Âge	51 years
Genre	Male
Origine ethnique	Asian
Morphologie	Epithelial
Propriétés de croissance	Adherent

Référence	KYSE-410 (Cytion catalog number 305122)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_1352

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Temps de doublement	32 to 45 hours
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305122-231023	Certificat d'analyse	23. May. 2025	305122

KYSE-410 Cells

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Certificat d'analyse (CoA)