SW-480 Cells
General information
Description | The SW480 cell line originated from a surgical specimen of a primary tumor of a moderately differentiated colon adenocarcinoma. |
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Organism | Human |
Tissue | Colon |
Disease | Adenocarcinoma, Grade IV, Dukes' type B. |
Synonyms | SW480, SW 480, SW480E |
Characteristics
Age | 51 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SW-480 (Cytion catalog number 300302) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | Epidermal growth factor (EGF), keratin (immunoperoxidase staining). Matrilysin, a metalloproteinase associated with tumor invasiveness, is not expressed. |
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Protein expression | The cells express elevated levels of p53 protein. |
Antigen expression | HLA A2, B8, B17, blood type A, Rh+. The line is negative for CSAp (CSAp-) and colon antigen 3 |
Isoenzymes | G6PD, B, PGM1, 2, PGM3, 1, 6PGD, A, PEP-D, 1, ES-D, 1 |
Tumorigenic | Yes, in nude mice |
Viruses | Reverse transcriptase negative |
Virus susceptibility | Human immunodeficiency virus (HIV, LAV) |
Products | Carcinoembryonic antigen (CEA) 0.7 ng/106 cells/10 days, keratin, TGF-?. The cells have been reported to produce GM-CSF. |
Mutational profile | SW-480 cells carry a homozygous Kras mutation in codon 12: GGT(Wt Gly) >GTT(Val). There is a G->A mutation in codon 273 of the p53 gene resulting in an Arg->His substitution and a C->T mutation in codon 309 resulting in a Pro->Ser substitution. |
Handling
Culture Medium | Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 20 to 25 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:8 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 1 to 2 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 13,14
D13S317: 12
D16S539: 13
D5S818: 13
D7S820: 8
TH01: 8
TPOX: 11
vWA: 16
D3S1358: 15
D21S11: 30,30.2
D18S51: 13
Penta E: 10
Penta D: 9,15
D8S1179: 13
FGA: 24
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HLA alleles |
A*: 02:01:01, 24:02:01
B*: 07:02:01, 15:18:01
C*: 07:02:01, 07:04:01
DRB1*: 01:03:01, 13:01:01
DQA1*: 01:01:01, 01:03:01
DQB1*: 05:01:01, 06:03:01
DPB1*: 01:01:01, 04:01:01
E: 01:01, 01:03
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