SW620 Cells
Basic details about the SW-620 cancer cell line
Description | The SW-620 cell line, originating from the large intestine of a 51-year-old male with Dukes-C colorectal cancer, is a pivotal research model in colorectal cancer, especially for cancer biomarkers, chemotherapy, and the study of metastatic cancer cells. SW-620 cells are pivotal for studying cell apoptosis and the resistance mechanisms to anoikis, a form of programmed cell death crucial for preventing metastasis. Research utilizing the SW-620 colon cancer cells has delved into proteomic analysis to understand proteome changes under different conditions, such as hypoxia. Hypoxic SW620 cells exhibit specific proteome adaptations that contribute to chemotherapy resistance. SW620 colon cancer cells have been pivotal in evaluating natural compounds like curcumin and their impact on cancer cell viability. Studies have shown that curcumin inhibits cell viability in SW-620 cells. Moreover, the cell line aids in assessing the effects of chemotherapeutic agents and the potential for chemotherapy resistance, which is critical for advancing cancer treatment strategies. Exhibiting high tumorigenic and metastatic capabilities, SW-620 cells form solid tumors in vivo. The SW620 xenograft model, alongside the study of specific pathways like the catenin pathway and the role of transcription factors such as cdx2 in colonic adenocarcinoma cells, enriches our understanding of colorectal cancer's molecular underpinnings. In summary, SW-620 human colon adenocarcinoma cells are an invaluable resource in cancer research, offering a multifaceted approach to understanding colorectal cancer's complexities. |
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Organism | Human |
Tissue | Colorectal |
Disease | Adenocarcinoma |
Metastatic site | Lymph node |
Synonyms | SW620, SW 620, SW.620 |
Aspects
Age | 51 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Specification
Citation | SW-620 (Cytion catalog number 300466) |
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Biosafety level | 1 |
Genetic makeup of the colorectal cancer cell line SW620
Tumorigenic | Yes, in athymic nude mice |
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Karyotype | Average number of chromosomes 48 (range, 46-52). Eighteen marker chromosomes. For a detailed description of the karyotype we refer to Melcher et al. |
Maintenance techniques
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 is recommended |
Fluid renewal | 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality assurance of SW620 cells
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 13,14
D13S317: 12
D16S539: 9,13
D5S818: 13
D7S820: 8,9
TH01: 8
TPOX: 11
vWA: 16
D3S1358: 16
D21S11: 30,30.2
D18S51: 13
Penta E: 10
Penta D: 9,15
D8S1179: 13
FGA: 24
D1S1656: 13,14
D6S1043: 11,12
D2S1338: 17,24
D12S391: 17
D19S433: 13
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HLA alleles |
A*: 02:01:01, 24:02:01
B*: 07:02:01, 15:18:01
C*: 07:02:01, 07:04:01
DRB1*: 01:03:01, 13:01:01
DQA1*: 01:01:01, 01:03:01
DQB1*: 05:01:01, 06:03:01
DPB1*: 01:01:01, 04:01:01
E: 01:01, 01:03
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