MDA-MB-435S Cells

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 300277

安全資料表

產品說明書

分析證明書 (CoA)

說明	Disclaimer: The cell line in question has been identified as problematic due to contamination issues. Specifically, the parent cell line (MDA-MB-435) has been shown to be a derivative of the M14 cell line. The MDA-MB-435S cell line is a widely utilized model in cancer research, originally thought to be derived from a breast cancer metastasis. These cells exhibit characteristics typical of highly aggressive cancer cells, including a rapid proliferation rate, resistance to apoptosis, and the ability to invade surrounding tissues. Due to these traits, MDA-MB-435S cells are frequently used in studies investigating cancer metastasis, drug resistance mechanisms, and the molecular underpinnings of aggressive tumor behavior. Interestingly, subsequent molecular and genetic analyses have revealed that MDA-MB-435S cells share a closer genetic profile with melanoma rather than breast cancer, raising significant implications for their use in research. Despite this controversy, they remain a valuable model for studying metastatic processes and testing potential therapeutic agents, particularly those targeting mechanisms common to both breast cancer and melanoma. Researchers are advised to consider these genetic findings when interpreting results obtained from studies involving MDA-MB-435S cells.
生物體	Human
組織	Skin
疾病	Amelanotic melanoma
轉移部位	Right buttock, hypodermis (primary melanoma/M14 origin)
應用	Metastasis and invasion research; melanoma/breast cancer controversy model; drug resistance mechanisms; tumor biology; preclinical pharmacological screening
同義詞	MDA-MB-435s, MDA-MB-435 S, MDA-MB-435-S, MDAMB435S, BrCL15

年齡	33 years
性別	Male
族裔	European
形態學	Epithelial-like
細胞類型	Epithelial cells
生長特性	Adherent

引用	MDA-MB-435S (Cytion catalog number 300277)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0622
轉基因狀態	No genetic modification; problematic line — parental MDA-MB-435 identified as M14 melanoma derivative; use with appropriate caution and cite genetic identity

培養基	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
營養補充品	Supplement the medium with 5% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
拆股比例	1 to 5
播種密度	1 to 3 × 10⁴ cells/cm²
流體更新	2 to 3 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.