DMS-114 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 305364

安全資料表

產品說明書

分析證明書 (CoA)

說明	DMS-114 is a human small-cell lung cancer (SCLC) cell line with unique features distinguishing it from other SCLC subtypes. Recent research has indicated that DMS-114, previously classified within the YAP1-expressing SCLC category (SCLC-Y), harbors pathogenic mutations in SMARCA4, an ATPase subunit of the SWI/SNF chromatin-remodeling complex. These mutations are associated with the absence of RB1 mutations, contrary to the typical mutational landscape of SCLC, which commonly features concurrent TP53 and RB1 alterations. This cell line's profile includes reduced expression of SMARCA4 mRNA and protein, contributing to its reclassification as a SMARCA4-deficient undifferentiated tumor (SMARCA4-UT) rather than a traditional SCLC. Morphological assessments have shown that DMS-114 aligns more closely with thoracic SMARCA4-UT, exhibiting traits such as lower neuroendocrine marker expression and a distinctive immunohistochemical profile. The revised classification of DMS-114 as a SMARCA4-deficient malignancy rather than SCLC has significant implications for its use as a preclinical model. It serves as an important resource for studying therapeutic strategies targeting SMARCA4-related pathways and investigating the biology of aggressive thoracic cancers that mimic SCLC. Unlike conventional SCLC, SMARCA4-deficient tumors, including DMS-114, often present with unique gene expression profiles marked by high YAP1 expression, loss of certain neuroendocrine markers, and distinct molecular vulnerabilities. This insight underscores the necessity of comprehensive molecular and histopathological analysis for accurate tumor classification and the development of effective treatment strategies.
生物體	Human
組織	Lung
疾病	Thoracic SMARCA4-deficient undifferentiated tumor
同義詞	DMS-114, DMS114, Darmouth Medical School 114

年齡	68 years
性別	Male
族裔	Caucasian
生長特性	Adherent

引用	DMS-114 (Cytion catalog number 305364)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_1174

受體的表達	Epidermal growth factor (EGF), complement (CR3)
蛋白質表達	Genes expressed: adrenocorticotropin (adrenocorticotropic hormone, ACTH), bombesin, glucagon, 17 beta estradiol, oxytocin - neurophysin (OT-NP)
抗原表達	Leu 7 +, My23 +, CD11b +
致瘤性	Yes, in nude mice
突變譜	Mutation: SMARCA4, p.Glu1310Ter (c.3928G>T), homozygous; Mutation: PARD3B, Ex2-14del, homozygous; Mutation: TP53, p.Arg213Ter (c.637C>T), homozygous

培養基	Waymouth's MB 752/1 medium (We do not supply this product; please consider other suppliers. Please let us know if you need further assistance)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
流體更新	2 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
305364-040425	分析證明書	23. May. 2025	305364

DMS-114 Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)