U-87 MG-RFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

1 104,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prix hors taxes, frais de livraison en sus

cryovial

Numéro de produit : 305702

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	U-87 MG-RFP cells are a fluorescently labeled variant of the human glioblastoma astrocytoma cell line U-87 MG, which was originally derived from a malignant glioma tumor of an adult patient. The parental U-87 MG line is widely used as an in vitro model for glioblastoma due to its well-characterized growth properties, tumorigenic potential, and relevance to high-grade astrocytic tumors. These cells exhibit adherent growth with epithelial-like morphology and are commonly employed to study processes such as cell proliferation, invasion, angiogenesis, and response to hypoxic conditions. In U-87 MG-RFP cells, stable expression of red fluorescent protein (RFP) enables real-time visualization of tumor cell behavior in both in vitro and in vivo systems. This modification facilitates applications such as live-cell imaging, tumor tracking in orthotopic xenograft models, and analysis of invasive growth patterns within brain tissue. U-87 MG-RFP cells are particularly valuable for studying glioblastoma progression, tumor–microenvironment interactions, and evaluating therapeutic strategies using fluorescence-based imaging approaches.
Organisme	Human
Tissu	Brain
Maladie	Glioblastoma
Synonymes	U-87MG, U87 MG, U-87-MG, U87-MG, U-87 MG, U-87, U87, 87 MG, 87MG

Âge	44 years
Genre	Male
Origine ethnique	Caucasian
Morphologie	Epithelial-like
Propriétés de croissance	Adherent

Référence	U87MG-RFP (Cytion catalog number 305702)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Statut OGM	GMO-S1: This human glioblastoma RFP-tagged cell line (U87MG-RFP) contains a lentiviral construct encoding red fluorescent protein from Aequorea victoria, enabling stable red fluorescence labeling. The modification is stably present. This classification applies only within Germany and may differ elsewhere.

Expression des protéines	RFP
Isoenzymes	Me-2, 1, PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B
Tumorigène	Yes, in nude mice inoculated subcutaneously with 107 cells

Milieu de culture	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Densité de semis	1.5 to 2 x 10⁴ cells/cm²
Milieu de congélation	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305702-170326	Certificat d'analyse	13. Apr. 2026	305702

U-87 MG-RFP Cells

Renseignements généraux

Caractéristiques

Données réglementaires

Données biomoléculaires

Manipulation

Contrôle de la qualité et analyse moléculaire

Certificat d'analyse (CoA)