Sf9 Cells (suspension)

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prix hors taxes, frais de livraison en sus

Propriétés de croissance

Adherent

Suspension

cryovial

Numéro de produit : 604328

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	Sf9 cells are clonal isolates derived from the Spodoptera frugiperda Sf21 cell line (IPLB-Sf-21-AE). They are commonly used in insect cell culture for recombinant protein production using baculovirus expression systems. Sf9 cells are epithelial in morphology and were cloned from the pupal ovarian tissue of the fall armyworm. One of the key characteristics of Sf9 cells is their small, regular size. They are also suitable for transfection, plaque assay/purification, amplification of high-titer stocks, and expression of recombinant proteins. The Sf9 insect cell line can be maintained in attached and suspended cultures, and do not require serum or CO2 to grow. They are considered Biosafety Level 1 and are usually grown in a 26-28 degree celsius incubator. Sf9 cells/baculovirus expression systems are widely used for high-level protein expression, often for purification, but proteins may also be functionally expressed in the defined Sf9 cell environment. The size of infected Sf9 cells is generally 17-30 microns in diameter. The Sf9 cell line is distinct from the Sf21 cell line in that it is a clonal isolate with a smaller and more regular size, while Sf21 cells are more disparate in size and form monolayers and plaques that are more irregular. Some Sf9 cell lines may harbor a negative sense Rhabdovirus called Spodoptera frugiperda rhabdovirus (SfRV), although not all tested Sf9 cells appear to be infected with this virus. The genome size of Sf9 has been estimated to be 451 Mbp with a G+C content of 36.53%.
Organisme	Fall armyworm
Tissu	Ovary
Applications	Transfection, plaque assay/purification, amplification of high-titer stocks, and expression of recombinant proteins
Synonymes	SF9, sf9, SF-9, Sf-9, sf-9, Sf 9, Spodoptera frugiperda clone 9, Sf clone 9, IPLB-Sf-9AE, IPLB-SF-9AE, IPLB-SF-9, IPLB-Sf-9, IPLB-Sf9

Âge	Pupal stage
Genre	Female
Morphologie	Round, attached, epitheloid
Propriétés de croissance	Suspension

Référence	Sf9 (Cytion catalog number 604328)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	7108
Cellosaurus - Numéro d'enregistrement	CVCL_0549

Sensibilité aux virus	Baculoviruses, Autographa californica (MNPV), St. Louis encephalitis (SLE)

Milieu de culture	Sf-900™ III SFM (Thermofisher, Cat-No. 12658019)
Suppléments	Supplement the medium with 0% FBS to enhance proliferation if needed
Réactif de dissociation	Accutase
Repiquage	Seeding after thawing 5x10⁵/ml. Incubate at 27°C-28°C, without CO₂, on a shaker with agitation of 90-110 rpm in Erlenmeyerflask. Keep at a cell density of 3-8x10⁵/ml. Maximum cell density possible is 1-4x10⁶ cells/ml.
Densité de semis	1 x 10⁴ cells/cm². Incubate between 26 to 30 degree Celsius in a non-humidified, ambient air-regulated incubator. Use cell culture flasks with filter caps or loosen caps to allow for oxygen exchange.
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete cell culture medium (including FBS) + 10% DMSO for adequate post-thaw viability.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	27°C, 0% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
604328-280725	Certificat d'analyse	22. Oct. 2025	604328

Sf9 Cells (suspension)

Renseignements généraux

Caractéristiques

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Données biomoléculaires

Manipulation

Contrôle de la qualité et analyse moléculaire

Certificat d'analyse (CoA)