RAW 264.7-GFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

1 104,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305699

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	RAW 264.7-GFP cells are a genetically modified derivative of the murine RAW 264.7 macrophage-like cell line, which originates from an adult mouse tumor of monocyte/macrophage lineage. These cells have been engineered to express green fluorescent protein (GFP), enabling real-time visualization of cellular processes such as morphology, migration, and phagocytic activity under fluorescence microscopy. The parental RAW 264.7 line is widely used as a model for innate immune responses, as it retains many functional characteristics of activated macrophages, including the ability to produce cytokines, nitric oxide, and reactive oxygen species upon stimulation. The incorporation of GFP allows for dynamic, non-invasive monitoring of macrophage behavior in vitro, making RAW 264.7-GFP cells particularly valuable for live-cell imaging studies and high-content screening applications. These cells respond robustly to pro-inflammatory stimuli such as lipopolysaccharide (LPS) and interferon-gamma, leading to activation of signaling pathways including NF-κB and MAPK. This makes them a useful model for investigating inflammatory signaling, host-pathogen interactions, and the effects of pharmacological agents on macrophage activation. However, as with the parental line, researchers should consider that RAW 264.7-derived models may exhibit altered regulatory pathways compared to primary macrophages.
Organisme	Mouse
Tissu	Ascites
Maladie	Mouse leukemia
Synonymes	GFP/RAW264.7

Âge	Adult
Genre	Male
Propriétés de croissance	Adherent

Référence	RAW 264.7-GFP (Cytion catalog number 305699)
Niveau de biosécurité	2
NCBI_Numéro d'identification fiscale	10090
Cellosaurus - Numéro d'enregistrement	CVCL_D7C8

Expression des protéines	GFP

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Densité de semis	2 to 5 x 10⁴ cells/cm²
Renouvellement des fluides	3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305699-260226	Certificat d'analyse	26. Mar. 2026	305699

RAW 264.7-GFP Cells

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Certificat d'analyse (CoA)