PC-9 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

759,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305045

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	The PC-9 cell line is derived from a human lung adenocarcinoma, a subtype of non-small cell lung cancer (NSCLC). This cell line is particularly notable for harboring an activating mutation in the EGFR gene, specifically the exon 19 deletion (E746_A750del), which is a common driver mutation in NSCLC. This alteration makes PC-9 an invaluable model for studying the biology of EGFR-driven cancers and evaluating the efficacy of tyrosine kinase inhibitors (TKIs) like gefitinib and erlotinib, which specifically target this pathway. PC-9 cells have been extensively used in research focused on resistance mechanisms to EGFR TKIs, particularly the emergence of secondary mutations like T790M. These studies have informed the development of third-generation inhibitors such as osimertinib, which target both the primary EGFR mutation and resistance-associated alterations. The cell line also exhibits sensitivity to other inhibitors targeting downstream signaling pathways, including those involved in PI3K/AKT and MAPK signaling cascades, underscoring its utility in translational cancer research. In addition to its genetic and pharmacological attributes, PC-9 has been incorporated into high-throughput drug screening programs, facilitating the identification of compounds with selective activity against EGFR-mutated NSCLC. The line's well-characterized genomic landscape and consistent phenotypic behavior in vitro make it a cornerstone for both basic and applied lung cancer research, particularly in the context of targeted and combination therapy.
Organisme	Human
Tissu	Lung
Maladie	Lung adenocarcinoma
Site métastatique	Lymph node
Synonymes	PC9, PC-9/S1, PC-9S1

Âge	45 years
Genre	Male
Morphologie	Heterogeneous mixture of round cells and spindle shaped cells
Propriétés de croissance	Adherent

Référence	PC-9 (Cytion catalog number 305045)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_B260

Tumorigène	Yes

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Repiquage	Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at 37°C for 10-15 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
Renouvellement des fluides	1 to 2 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305045-211122	Certificat d'analyse	23. May. 2025	305045
305045-110825	Certificat d'analyse	22. Oct. 2025	305045

PC-9 Cells

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Certificat d'analyse (CoA)