MKN-7 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

897,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305104

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Certificat d'analyse (CoA)

Description	The MKN-7 cell line is a well-characterized human gastric carcinoma cell line, established from a well-differentiated tubular adenocarcinoma. This cell line is part of a broader panel of gastric cancer cell lines that were developed to study the varied histological and biological behaviors of gastric carcinomas. MKN-7 cells are known to display morphological characteristics indicative of intestinal differentiation, such as cell polarity and the presence of microvilli with core filaments. These features are typically observed in both in vitro cultures and in xenografts in nude mice, although the degree of differentiation may diminish over time with prolonged culture conditions. In terms of functional characteristics, MKN-7 cells exhibit low fibrinolytic activity, which is primarily plasminogen-dependent. This activity is significantly lower compared to other gastric cancer cell lines like MKN-1 and MKN-28, which show higher fibrinolytic activities. The low fibrinolytic activity of MKN-7 cells may be relevant in studies investigating the role of fibrinolysis in cancer progression, particularly in relation to the invasive and metastatic potential of gastric tumors. Furthermore, the MKN-7 cell line, along with other gastric cancer cell lines, has been utilized in studies examining thromboplastic activity, though MKN-7 is noted for its relatively low levels of this activity as well. This suggests a more limited role in the hypercoagulable states often associated with aggressive tumor phenotypes.
Organisme	Human
Tissu	Stomach
Maladie	Gastric tubular adenocarcinoma
Site métastatique	Lymph node
Synonymes	MKN-7, MKN 7

Âge	39 years
Genre	Female
Origine ethnique	Asian
Morphologie	Epithelial
Propriétés de croissance	Adherent

Référence	MKN-7 (Cytion catalog number 305104)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_1417

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305104-170425	Certificat d'analyse	21. Jul. 2025	305104
305104-271123	Certificat d'analyse	23. May. 2025	305104

MKN-7 Cells

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Certificat d'analyse (CoA)