KC Cells
Renseignements généraux
| Description | KC (Kc167) is a Drosophila melanogaster embryonic cell line derived from a dorsal closure stage female embryo, registered in Cellosaurus as CVCL_Z833. The line was originally established and has been widely used in Drosophila cell biology, genetic screens, and functional genomics. Kc167 cells have been immortalized with SV40 and grow semi-adherently, forming a mixed population of attached and loosely floating cells. They are among the best-characterized Drosophila cell lines and are fully amenable to RNAi-mediated gene silencing, making them a cornerstone model for genome-wide RNAi screens in invertebrate biology. KC cells are applicable in Drosophila cell biology, genome-wide RNAi and CRISPR screens, signal transduction pathway studies (Wnt/Wingless, Hedgehog, Notch, JAK/STAT, Toll/NF-κB), innate immunity research, and comparative studies of evolutionarily conserved pathways. The fully sequenced and annotated Drosophila genome, combined with extensive public databases of RNAi reagents (e.g., DRSC/TRiP), makes KC cells particularly powerful for unbiased functional genomic discovery. BSL-2 classification applies due to the SV40 immortalization. KC cells are cultured in Schneider's Drosophila medium supplemented with 2 mM Glutamine and 10% FBS. Critically, Drosophila cell lines are cultured at 25°C in ambient air without CO₂ (not 37°C / 5% CO₂ as for mammalian cells). Cells are passaged by gentle resuspension and dilution (semi-adherent growth). Medium is renewed or cells are diluted every 2–3 days. |
|---|---|
| Organisme | Drosophila melanogaster (Fruit fly) |
| Tissu | Embryo |
| Maladie | Normal |
| Site métastatique | Embryo (dorsal closure stage) |
| Applications | Drosophila cell biology; genome-wide RNAi screens; CRISPR screens; signal transduction (Wnt/Wingless, Hedgehog, Notch, JAK/STAT, Toll/NF-κB); innate immunity; invertebrate functional genomics |
| Synonymes | KC, K C |
Caractéristiques
| Âge | Dorsal closure stage |
|---|---|
| Genre | Female |
| Morphologie | Semi-adherent epithelial-like |
| Type de cellule | Embryonic cells |
| Propriétés de croissance | semi-adherent |
Données réglementaires
| Référence | KC (Cytion catalog number 300604) |
|---|---|
| Niveau de biosécurité | 2 |
| NCBI_Numéro d'identification fiscale | 7227 |
| Cellosaurus - Numéro d'enregistrement | CVCL_Z833 |
| Statut OGM | GMO-S2: This Drosophila cell line contains a stably integrated SV40 immortalization cassette. BSL-2 containment is required. This classification applies only within Germany and may differ elsewhere. |
Données biomoléculaires
| Sensibilité aux virus | SV40 immortalized |
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Manipulation
| Milieu de culture | Schneider's Drosophila medium + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
|---|---|
| Suppléments | Supplement the medium with 2 mM Glutamine and 10% FBS |
| Réactif de dissociation | Not required (semi-adherent; resuspend gently) |
| Temps de doublement | approx. 24 to 48 hours |
| Repiquage | Gently pipette the culture to resuspend semi-adherent cells. Transfer an appropriate volume to a new flask and add fresh pre-warmed Schneider's medium. Dilute to a density of 5 × 10⁵ to 1 × 10⁶ cells/ml. Incubate at 25°C in ambient air without CO₂. |
| Rapport de fractionnement | 1 to 3 |
| Densité de semis | 5 × 10⁵ to 1 × 10⁶ cells/ml |
| Renouvellement des fluides | Every 2 to 3 days |
| Milieu de congélation | As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. |
| Décongélation et culture des cellules |
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| Atmosphère d'incubation | 25°C, ambient air (no CO₂ required). |
| Conditions d'expédition | Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage. |
| Conditions d'entreposage | For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen. |