K-562-GFP Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

1 104,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305948

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	K-562-GFP cells are a genetically modified variant of the human chronic myelogenous leukemia (CML) cell line K-562, originally derived from the peripheral blood of an adult patient in blast crisis. The parental K-562 line is characterized by the presence of the Philadelphia chromosome, resulting in the BCR-ABL fusion protein with constitutive tyrosine kinase activity, which drives uncontrolled proliferation and survival. K-562 cells exhibit erythroleukemia features and can be induced to undergo differentiation along erythroid, megakaryocytic, or monocytic lineages under specific experimental conditions, making them a versatile model for studying hematopoietic differentiation and leukemia biology. The introduction of green fluorescent protein (GFP) into K-562 cells enables real-time visualization and tracking of leukemic cell behavior in vitro and in vivo. K-562-GFP cells are widely used in assays involving cell proliferation, migration, and drug response, as well as in co-culture systems to study interactions with stromal or immune cells. The fluorescent labeling facilitates applications such as flow cytometry, live-cell imaging, and high-throughput screening.
Organisme	Human
Tissu	Pleural effusion
Maladie	Chronic myeloid leukemia

Âge	53 years
Genre	Female
Origine ethnique	Caucasian
Morphologie	Lymphoblast-like
Type de cellule	Lymphoblast
Propriétés de croissance	Suspension

Référence	K562-GFP (Cytion catalog number 305948)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_1G55

Expression des protéines	GFP
Profil mutationnel	Mutation: p.Gln136fs*13, Homozygous

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	None
Repiquage	Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 10⁵ cells/ml and keep the cell concentration within the range of 3 x 10⁵ to 1 x 10⁶ cells/ml for optimal growth.
Densité de semis	0.3 to 1 x 10⁶ cells/ml
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305948-120326	Certificat d'analyse	13. Apr. 2026	305948

K-562-GFP Cells

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Certificat d'analyse (CoA)