IM-9 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 302151

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Certificat d'analyse (CoA)

Description	IM-9 is a human lymphoblastoid cell line established in 1967 from the bone marrow of an adult woman diagnosed with multiple myeloma. Originally believed to be derived from myeloma cells, subsequent research, including findings published by Pellat-Deceunynk et al. in 1995, has indicated that IM-9 cells are more accurately classified as Epstein-Barr Virus-positive (EBV+) B-lymphoblastoid cells rather than malignant myeloma cells. This distinction is crucial for researchers using this cell line, as it affects the interpretation of experimental outcomes related to myeloma studies. IM-9 cells have been extensively characterized in the literature and are noted for synthesizing Immunoglobulin G (IgG). They are also known to express receptors for insulin and calcitonin, making them valuable for studying hormone-receptor interactions. Additionally, these cells express BCL2 mRNA, a gene involved in regulating apoptosis, which is often studied in the context of cancer and immune cell survival. Due to their high expression of insulin receptors, IM-9 cells are frequently used in research focused on insulin signaling and metabolic disorders, providing insights into insulin resistance mechanisms. The IM-9 cell line remains a significant resource for various research applications, particularly in the fields of immunology, cancer biology, and metabolic studies. However, given the revised understanding of their origin, it is critical to use IM-9 cells with the recognition that they are not representative of malignant myeloma cells. As always, these cells are intended exclusively for in vitro research and are not suitable for therapeutic or in vivo use.
Organisme	Human
Tissu	Bone marrow
Synonymes	IM 9, IM9, GM04680

Âge	Unspecified
Genre	Female
Origine ethnique	Caucasian
Morphologie	Round cells in cluster
Type de cellule	B lymphoblast
Propriétés de croissance	Suspension

Référence	IM-9 (Cytion catalog number 302151)
Niveau de biosécurité	2
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_1305

Expression de l'antigène	CD19+, CD20+, CD23+, CD27+, CD80+, CD83+, CD138+, MHC I+, MHC II+
Virus	EBV+ free of human pathogenic viruses SV40, JC/BK, HBV, HCV, HIV.

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% heat-inactivated FBS
Repiquage	Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10⁵ cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
Renouvellement des fluides	2 to 3 times per week
Rétablissement après le dégel	Fast
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
302151-270225	Certificat d'analyse	23. May. 2025	302151

IM-9 Cells

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Certificat d'analyse (CoA)