HEL 92.1.7 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 300462

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Certificat d'analyse (CoA)

Description	The HEL 92.1.7 cell line exhibits the capacity for spontaneous differentiation into erythroblast-like cells, mimicking some aspects of erythroid maturation in vitro. This characteristic makes them particularly useful for studying the erythroid differentiation process and the regulation of gene expression related to erythropoiesis. Their ability to spontaneously differentiate offers a unique advantage for studying the intrinsic pathways and mechanisms that drive erythroid precursor maturation without the addition of external differentiation-inducing agents. Moreover, the differentiation of HEL 92.1.7 cells can be further manipulated through the addition of phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid), which are known to induce macrophage-like differentiation. This induced differentiation into macrophage-like cells expands the utility of the HEL 92.1.7 cell line beyond erythroid studies, allowing researchers to explore and understand the plasticity of hematopoietic cells and the conditions under which lineage commitment and cellular identity can be redirected. Such studies are crucial for developing therapeutic strategies aimed at manipulating cell fate for regenerative medicine and cancer treatment.
Organisme	Human
Tissu	Bone marrow
Maladie	Erythroleukemia
Synonymes	HEL92.1.7, HEL-92.1.7, HEL-92-1-7, HEL-92_1_7, HEL-92, HEL92

Âge	30 years
Genre	Male
Origine ethnique	Caucasian
Morphologie	Round cells
Type de cellule	Erythroblast
Propriétés de croissance	Suspension

Référence	HEL 92.1.7 (Cytion catalog number 300462)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_2481

Expression de l'antigène	HLA A3, Aw32, Bw35, Ia+
Produits	Hemoglobin, globin (G gamma, A gamma, epsilon, zeta and alpha chains), beta-2-microglobulin, glycophorin

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% heat-inactivated FBS
Réactif de dissociation	Accutase
Repiquage	Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
300462-090425	Certificat d'analyse	23. May. 2025	300462
300462-190525	Certificat d'analyse	21. Jul. 2025	300462

HEL 92.1.7 Cells

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Certificat d'analyse (CoA)