HEK293-CLDN18.2 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

2 622,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305986

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Description	Disclaimer: The prices displayed for cell lines are exclusively for academic/not-for-profit customers. For commercial entitites the price is approximately €6,250. If you represent a commercial entity or are unsure which category applies, please contact us. HEK293-CLDN18.2 cells are human embryonic kidney 293 (HEK293) cells engineered to stably express human claudin 18 isoform 2 (CLDN18.2), a tight junction-associated transmembrane protein belonging to the claudin family. CLDN18.2 is a gastric lineage-specific isoform normally restricted to differentiated gastric mucosal epithelial cells, where its extracellular domains are largely inaccessible under physiological conditions. In malignant transformation, disruption of epithelial polarity and tight junction architecture exposes CLDN18.2 on the tumor cell surface, leading to its overexpression and accessibility in several cancers, including gastric adenocarcinoma, gastroesophageal junction cancer, pancreatic cancer, and other gastrointestinal malignancies. Because of its highly restricted normal tissue distribution and tumor-associated exposure, CLDN18.2 has emerged as a clinically important therapeutic target in oncology. HEK293-CLDN18.2 cells are widely used for development and characterization of CLDN18.2-targeted therapeutics, including monoclonal antibodies, antibody-drug conjugates, bispecific antibodies, CAR-T and CAR-NK cell therapies, and targeted imaging agents. The stable recombinant expression system enables quantitative analysis of antigen binding affinity, epitope specificity, receptor density, internalization kinetics, and target-dependent cytotoxicity. These cells are also commonly employed in flow cytometry assays, reporter assays, antibody screening workflows, and immune effector functional studies designed to evaluate antibody-dependent cellular cytotoxicity (ADCC) or complement-dependent cytotoxicity (CDC). Because HEK293 cells support robust recombinant membrane protein expression and efficient propagation, they provide a reliable platform for standardized CLDN18.2 assay development and therapeutic validation.
Organisme	Human
Tissu	Fetal Kidney
Maladie	Transformed/immortalized; non-tumorigenic (HEK293 background)
Applications	CLDN18.2-targeted antibody and ADC development; CAR-T/CAR-NK cell therapy; ADCC/CDC assays; flow cytometry; gastric/GEJ/pancreatic cancer therapeutics

Âge	Fetus
Genre	Female
Morphologie	Epithelial-like
Type de cellule	Epithelial cells
Propriétés de croissance	Monolayer, adherent

Référence	HEK293-CLDN18.2 (Cytion catalog number 305986)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_E5J2
Statut OGM	GMO-S1: This HEK293 cell line contains a CLDN18.2 expression construct for tight junction antigen studies and targeted cancer therapy development. This classification applies only within Germany and may differ elsewhere.

Antigènes de surface	CLDN18.2
Récepteurs exprimés	CDLN18.2

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS, 1 mM sodium pyruvate, 10 mM HEPES, 1% NEAA. Add Geneticin (G418-Sulfat) to achieve a final concentration of 1 mg/mL.
Réactif de dissociation	Trypsin-EDTA
Temps de doublement	approx. 24-36 hours
Repiquage	For routine adherent cell culture: Aspirate the old culture medium from the adherent cells, and wash them with PBS to remove any remaining medium. After aspirating the PBS, add the appropriate volume of Trypsin/EDTA solution based on the culture vessel size (e.g., 1 ml for a T25 flask, 3 ml for a T75 flask) and incubate at room temperature or 37°C until the cells detach (5-10 minutes). Monitor detachment under a microscope, and gently tap the vessel if necessary to release the cells. Once detached, add complete medium to inactivate the Trypsin/EDTA, gently resuspend the cells, and transfer an aliquot of the cell suspension into a new culture vessel containing fresh medium. Place the vessel in an incubator set to 37°C with 5% CO₂, and change the medium every 2-3 days.
Rapport de fractionnement	1 to 5
Densité de semis	2 to 4 x 10⁴ cells/cm²
Renouvellement des fluides	2 to 3 times per week
Rétablissement après le dégel	After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

HEK293-CLDN18.2 Cells

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