HCC4006 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305785

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	HCC4006 is a human non-small cell lung cancer (NSCLC) cell line derived from a lung adenocarcinoma. It is characterized by an activating exon 19 deletion in the EGFR gene, which makes it particularly sensitive to EGFR tyrosine kinase inhibitors (TKIs) such as erlotinib and gefitinib. This feature has made HCC4006 a widely used model for studying EGFR-mutant NSCLC and resistance mechanisms to EGFR-targeted therapies. In the Cancer Cell Line Encyclopedia (CCLE), HCC4006 has been comprehensively profiled at the genomic, transcriptomic, and epigenetic levels, confirming its high sensitivity to EGFR inhibition and highlighting its use as a pharmacogenomic reference model. High-resolution genomic studies have revealed that HCC4006 displays a relatively simple karyotype compared to other NSCLC models, which may facilitate clearer interpretation of drug responses and genomic alterations. It lacks common resistance mutations such as T790M in the EGFR gene, making it suitable for modeling initial treatment responses. However, resistance can be induced in vitro, allowing researchers to study mechanisms of acquired resistance. For example, resistance to EGFR TKIs in HCC4006 has been linked to epithelial-mesenchymal transition (EMT) and activation of alternative signaling pathways, such as AXL kinase overexpression. HCC4006 has also been assessed in large-scale transcriptomic comparisons of cell lines and primary tumors. It is one of the lung adenocarcinoma cell lines that demonstrates a moderate correlation to primary tumor gene expression profiles, though the degree of correlation can vary depending on the purity of the tumor samples used for comparison. These analyses underscore the relevance of HCC4006 in modeling certain molecular aspects of lung adenocarcinoma, particularly those associated with EGFR-driven oncogenesis, while also emphasizing its limitations in fully recapitulating the heterogeneity of primary tumors.
Organisme	Human
Tissu	Metastatic
Maladie	Lung adenocarcinoma
Site métastatique	Pleural effusion
Synonymes	HCC-4006, Hamon Cancer Center 4006

Âge	>50 years
Genre	Male
Origine ethnique	Caucasian
Morphologie	Epithelial
Type de cellule	Epithelial cell
Propriétés de croissance	Adherent

Référence	HCC4006 (Cytion catalog number 305785)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_1269

Profil mutationnel	Mutation: EGFR, Simple, p.Leu747_Glu749del (c.2239_2247delTAAGAGAA), Heterozygous (ATCC=CRL-2871, TP53, Simple, p.Tyr205His (c.613T>C), Homozygous (DepMap=ACH-000066).

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Temps de doublement	46 hours
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305785-040825	Certificat d'analyse	22. Oct. 2025	305785

HCC4006 Cells

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Certificat d'analyse (CoA)