Colo-320DM Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 300153

Fiche signalétique

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Certificat d'analyse (CoA)

Description	The COLO-320DM cell line is a human colorectal adenocarcinoma cell line established from the metastatic site of a 55-year-old Caucasian female. This cell line exhibits unique characteristics that are significant for the study of colorectal cancer metastasis and the effects of chemotherapeutic agents. It is noteworthy for its high expression of carcinoembryonic antigen (CEA), a valuable biomarker used in the monitoring and diagnosis of colorectal cancer. COLO-320DM cells are adherent with an epithelial-like morphology. They are often used in research focused on the cellular and molecular mechanisms underlying colorectal cancer progression and metastasis. In addition, due to their consistent growth patterns and genetic stability over passages, they serve as a reliable model for in vitro experiments investigating cancer cell biology, drug response, and gene expression related to colorectal cancer. These cells also present a particular interest for genetic studies, especially those related to the pathways involved in metastasis and response to chemotherapy. Researchers utilize COLO-320DM to explore signaling pathways, cellular response to hypoxia, and interactions between cancer cells and the tumor microenvironment. The cell line has been instrumental in the development of therapeutic strategies targeting metastatic mechanisms specific to colorectal carcinoma.
Organisme	Human
Tissu	Colon, Dukes' type C
Maladie	Colorectal adenocarcinoma
Synonymes	COLO_320DM, COLO-320-DM, COLO #320DM, COLO320/DM, COLO320-DM, COLO320DM, Colo320DM, COLO320 DM, COLO 320 DM, COLO 320 (DM), Colorado 320 Double Minutes

Âge	55 years
Genre	Female
Origine ethnique	Caucasian
Morphologie	Rounded and refractile
Propriétés de croissance	Adherent

Référence	COLO-320DM (Cytion catalog number 300153)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_0219

Isoenzymes	PGM1,1, PGM3, 2, G6PD, B, PEP-D, 1, 6PGD, A, ES-D, 1
Tumorigène	Yes, in nude mice
Produits	Serotonin, norepinephrine, epinephrine, adrenocorticotropic hormone (ACTH), parathyroid hormone

Milieu de culture	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densité de semis	1 x 10⁴ cells/cm²
Renouvellement des fluides	Every 3 to 5 days
Rétablissement après le dégel	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
300153-514SF	Certificat d'analyse	23. May. 2025	300153
300153-131125	Certificat d'analyse	08. Jan. 2026	300153

Colo-320DM Cells

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Certificat d'analyse (CoA)