CMT-93 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

593,40 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305761

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	The CMT93 cell line is a murine colorectal carcinoma model established from a chemically induced rectal tumor in a C57BL/ICRF mouse. The tumor originated following exposure to methylazoxymethanol acetate (MAM acetate), a potent carcinogen. CMT93 was derived from the fourth in vivo transplant generation of the original tumor and cultured using selective trypsinization techniques to isolate epithelial populations while removing mesenchymal contaminants. Through repeated subculturing and purification, the resulting cell line exhibited a stable epithelial morphology and growth pattern. In vitro, CMT93 cells grow in coherent epithelial clumps and exhibit hallmark features of differentiated intestinal epithelial cells. Electron microscopy revealed the presence of microvilli with glycoprotein strands, desmosomal junctions, and occasional acinus-like structures, suggesting partial retention of normal rectal epithelial architecture. These cells are highly adherent and grow to confluence in approximately 7 days following a 1:2 split. While predominantly epithelial, early passages included mixed populations, which were resolved through sequential selective subculturing. The line has been maintained for numerous passages and cryopreserved successfully for long-term use. CMT93 is widely used in gastrointestinal cancer research, particularly in studies exploring colorectal carcinogenesis, epithelial-mesenchymal interactions, immune responses, and microbial-host interactions. It is also featured in short tandem repeat (STR) profiling studies to support intraspecies authentication of mouse cell lines, confirming its unique identity and value as a validated model in preclinical research.
Organisme	Mouse
Tissu	Rectum
Maladie	Mouse rectum carcinoma
Synonymes	CMT-93, CMT 93, C57 Mouse Tumor 93

Race/Sous-espèce	C57BL/icrf
Âge	1 year 7 months
Genre	Male
Type de cellule	Epithelial
Propriétés de croissance	Adherent

Référence	CMT-93 (Cytion catalog number 305761)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	10090
Cellosaurus - Numéro d'enregistrement	CVCL_1986

Profil mutationnel

Milieu de culture	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Densité de semis	1 to 3 x 10⁴ cells/cm²
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305761-260326	Certificat d'analyse	15. May. 2026	305761

CMT-93 Cells

Renseignements généraux

Caractéristiques

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Données biomoléculaires

Manipulation

Contrôle de la qualité et analyse moléculaire

Certificat d'analyse (CoA)