A375-GFP

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

1 104,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305665

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	A375-GFP is a genetically engineered variant of the human malignant melanoma cell line A375, stably expressing enhanced green fluorescent protein (eGFP). The parental A375 cell line is derived from a skin melanoma tumor in an adult patient and is widely used as a model for cutaneous melanoma, particularly for studies involving oncogenic BRAF signaling, as it harbors the BRAF V600E mutation. This mutation leads to constitutive activation of the MAPK/ERK pathway, driving proliferation and survival, and making A375 cells highly relevant for investigating targeted therapies such as BRAF and MEK inhibitors. The GFP-expressing derivative retains these molecular and phenotypic characteristics while enabling fluorescence-based applications. The stable incorporation of the eGFP reporter allows for real-time visualization of A375-GFP cells in both in vitro and in vivo systems. Fluorescence imaging facilitates monitoring of cell proliferation, migration, invasion, and morphological changes, as well as tracking tumor growth and metastatic dissemination in xenograft models. The enhanced GFP variant provides improved brightness and stability compared to earlier GFP constructs, enabling sensitive detection even at low cell numbers. This makes A375-GFP particularly useful in co-culture experiments, high-content imaging platforms, and studies requiring precise spatial resolution of tumor cell behavior. A375-GFP maintains the aggressive and proliferative phenotype of the parental melanoma line, including responsiveness to MAPK pathway inhibitors and the capacity for invasion and metastasis in experimental models. The addition of GFP expands its utility for drug screening, live-cell imaging, and tumor–microenvironment interaction studies. As with other reporter-labeled cell lines, validation of fluorescence stability and consistency across passages is recommended for specific experimental applications.
Organisme	Human
Tissu	Leg, skin
Maladie	Amelanotic melanoma

Âge	54 years
Genre	Female
Origine ethnique	Caucasian
Propriétés de croissance	Adherent

Référence	A375-GFP (Cytion catalog number 305665)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_QZ67
Statut OGM	GMO-S1: This human A375 melanoma line contains an enhanced GFP expression construct delivered by lentiviral vector for fluorescent visualization. This classification applies only within Germany and may differ elsewhere.

Profil mutationnel	Mutation: BRAF, Simple, p.Val600Glu (c.1799T>A), Homozygous (from parent cell line).Mutation, CDKN2A, Simple, p.Glu61Ter (c.181G>T) (p.Gly75Val, c.224G>T), Homozygous (from parent cell line).Mutation, CDKN2A, Simple, p.Glu69Ter (c.205G>T) (p.Gly83Val, c.248G>T), Homozygous (from parent cell line).Mutation, TERT, Simple, c.1-146C>T (c.250C>T) (C250T), Unspecified, Note=In promoter (from parent cell line).

Milieu de culture	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densité de semis	1 to 3 x 10⁴ cells/cm²
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305665-200426	Certificat d'analyse	24. Jun. 2026	305665

A375-GFP

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Certificat d'analyse (CoA)