A2780-GFP

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

1 104,00 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305676

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	A2780-GFP is a genetically modified derivative of the human ovarian carcinoma cell line A2780, engineered to stably express green fluorescent protein (GFP). The parental A2780 cell line was established from an ovarian tumor in an adult patient and is widely used as a model for epithelial ovarian cancer, particularly in studies of chemotherapy response. It is known for its relative sensitivity to platinum-based agents such as cisplatin, making it a valuable system for investigating mechanisms of drug action and resistance. The GFP-expressing variant preserves these biological characteristics while incorporating a fluorescent reporter for enhanced experimental utility. The stable expression of GFP enables real-time visualization and quantitative analysis of A2780-GFP cells in a variety of experimental settings. Fluorescence-based detection allows researchers to monitor cell proliferation, morphology, migration, and viability in vitro, as well as to track tumor growth and dissemination in vivo. This is particularly advantageous in xenograft and metastasis models, where GFP facilitates discrimination of tumor cells from surrounding host tissue. The fluorescent signal is generally stable across passages, although expression levels may vary depending on the transduction method and clonal selection. A2780-GFP retains the core molecular and phenotypic features of the parental A2780 line, including pathways associated with DNA damage response, apoptosis, and chemotherapeutic sensitivity. As such, it is commonly used in high-content imaging assays, drug screening platforms, and co-culture systems where spatial and temporal resolution of tumor cell behavior is required. The addition of GFP significantly enhances the versatility of this model, supporting applications in ovarian cancer research, therapeutic evaluation, and studies of tumor cell dynamics.
Organisme	Human
Tissu	Ovary
Maladie	Ovarian endometrioid adenocarcinoma
Site métastatique	Primary tumor site (ovary)
Applications	Ovarian cancer imaging; GFP-based tumor tracking; in vivo fluorescence imaging; combination with parental A2780 for comparative studies; drug efficacy visualization

Âge	Age unspecified
Genre	Female
Origine ethnique	African American
Morphologie	Epithelial-like
Type de cellule	Epithelial cells
Propriétés de croissance	Adherent

Référence	A2780-GFP (Cytion catalog number 305676)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	Not assigned (A2780-GFP reporter derivative; parental A2780 CVCL_1099)
Statut OGM	GMO-S1: This human A2780 ovarian cancer line contains a lentiviral GFP expression construct for fluorescence-based imaging. This classification applies only within Germany and may differ elsewhere.

Expression de l'antigène	GFP

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Rapport de fractionnement	1 to 5
Densité de semis	1 to 3 × 10⁴ cells/cm²
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305676-040326	Certificat d'analyse	08. Apr. 2026	305676

A2780-GFP

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Certificat d'analyse (CoA)