5637 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 300105

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Fiche de produit

Certificat d'analyse (CoA)

Description	5637 is a bladder carcinoma cell line isolated from the urinary bladder of a 68-year-old man with grade II carcinoma. 5637 cells produce and secrete several growth factors, such as SCF, IL-1, IL-6, G-CSF, and GM-CSF. These cytokines are functionally active and can be a valuable source for the culture of growth factor-responsive or dependent hematopoietic primary cells and cell lines. The karyotype modal chromosome number of 5637 cells is 67, ranging from 59 to 71. The stemline modal chromosome number is 67 at 36% and polyploidy at 0.6%. Fourteen marker chromosomes are common to these cells, including 3q+, 11q+, i(13q), t(9q21q), i(17q), i(21q). Additional markers, like der(5)t(5;7)(q31;p11) and 1p, were only found specific to a minor subpopulation, as well as microchromosomes and double minutes (DM). Some cells include one or occasionally two Y chromosomes. 5637 cells are tumorigenic and have been shown to induce tumours in nude mice inoculated subcutaneously. The doubling time of 5637 cells is approximately 24 hours. The isoenzyme profile of 5637 cells consists of isoform 1 of AK-1, ES-D, Me-2 and PGM1, isoform 1 and 2 of GLO-I, isoform B of G6PD, as well as isoform 2 of PGM3. In terms of oncogenes, 5637 cells are positive for FGFR3, PIK3CA, HRAS, KRAS, NRAS, TERT, and CDKN2A but negative for TP53 and belong to the molecular bladder cancer subtype l5637 is a bladder carcinoma cell line isolated from the urinary bladder of a 68-year-old man with grade II carcinoma. 5637 cells produce and secrete several growth factors, such as SCF, IL-1, IL-6, G-CSF, and GM-CSF. These cytokines are functionally active and can be a valuable source for the culture of growth factor-responsive or dependent hematopoietic primary cells and cell lines. The karyotype modal chromosome number of 5637 cells is 67, ranging from 59 to 71. The stemline modal chromosome number is 67 at 36% and polyploidy at 0.6%. Fourteen marker chromosomes are common to these cells, including 3q+, 11q+, i(13q), t(9q21q), i(17q), i(21q). Additional markers, like der(5)t(5;7)(q31;p11) and 1p, were only found specific to a minor subpopulation, as well as microchromosomes and double minutes (DM). Some cells include one or occasionally two Y chromosomes. 5637 cells are tumorigenic and have been shown to induce tumours in nude mice inoculated subcutaneously. The doubling time of 5637 cells is approximately 24 hours. The isoenzyme profile of 5637 cells consists of isoform 1 of AK-1, ES-D, Me-2 and PGM1, isoform 1 and 2 of GLO-I, isoform B of G6PD, as well as isoform 2 of PGM3. In terms of oncogenes, 5637 cells are positive for FGFR3, PIK3CA, HRAS, KRAS, NRAS, TERT, and CDKN2A but negative for TP53 and belong to the molecular bladder cancer subtype luminal. In conclusion, 5637 cells are a valuable tool for cancer research, especially with respect to the study of growth factors, cell division, oncogenes, and bladder cancer.
Organisme	Human
Tissu	Bladder
Maladie	Carcinoma
Site métastatique	Primary tumor site (urinary bladder)
Applications	Bladder cancer research; hematopoietic growth factor production (SCF, IL-1, IL-6, G-CSF, GM-CSF); oncogene studies (FGFR3, PIK3CA, HRAS, KRAS, TERT); drug sensitivity; transfection host; feeder cells for hematopoietic cultures

Âge	68 years
Genre	Male
Origine ethnique	Caucasian
Morphologie	Epithelial-like
Type de cellule	Epithelial cells
Propriétés de croissance	Adherent

Référence	5637 (Cytion catalog number 300105)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_0126
Statut OGM	No genetic modification; wildtype bladder carcinoma cell line

Isoenzymes	Me-2, 1, PGM3, 2, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B
Tumorigène	Yes, In nude mice.
Produits	IL-1, IL-6, G-CFS, GM-CSF, SCF
Statut de ploïdie	The modal chromosome number of the stemline cells is 67, comprising 36% of the total. Polyploidy occurs in 0.6% of these cells. Each cell typically had one or occasionally two Y chromosomes.
Caryotype	Phenotype Frequency Product: 0.0056.

Milieu de culture	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Temps de doublement	24 hours
Repiquage	First, remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Rapport de fractionnement	1 to 5
Densité de semis	1 x 10⁴ cells/cm² will result in a confluent monolayer within 3 days.
Renouvellement des fluides	2 to 3 times per week
Rétablissement après le dégel	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
300105-140425	Certificat d'analyse	23. May. 2025	300105
300105-715SF	Certificat d'analyse	23. May. 2025	300105
300105-613	Certificat d'analyse	05. Dec. 2025	300105

5637 Cells

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Certificat d'analyse (CoA)