A2780-GFP

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD 800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Los precios no incluyen impuestos ni gastos de envío

cryovial

Número de producto: 305676

Ficha de datos de seguridad

Ficha del producto

Certificado de análisis (CoA)

Descripción	A2780-GFP is a genetically modified derivative of the human ovarian carcinoma cell line A2780, engineered to stably express green fluorescent protein (GFP). The parental A2780 cell line was established from an ovarian tumor in an adult patient and is widely used as a model for epithelial ovarian cancer, particularly in studies of chemotherapy response. It is known for its relative sensitivity to platinum-based agents such as cisplatin, making it a valuable system for investigating mechanisms of drug action and resistance. The GFP-expressing variant preserves these biological characteristics while incorporating a fluorescent reporter for enhanced experimental utility. The stable expression of GFP enables real-time visualization and quantitative analysis of A2780-GFP cells in a variety of experimental settings. Fluorescence-based detection allows researchers to monitor cell proliferation, morphology, migration, and viability in vitro, as well as to track tumor growth and dissemination in vivo. This is particularly advantageous in xenograft and metastasis models, where GFP facilitates discrimination of tumor cells from surrounding host tissue. The fluorescent signal is generally stable across passages, although expression levels may vary depending on the transduction method and clonal selection. A2780-GFP retains the core molecular and phenotypic features of the parental A2780 line, including pathways associated with DNA damage response, apoptosis, and chemotherapeutic sensitivity. As such, it is commonly used in high-content imaging assays, drug screening platforms, and co-culture systems where spatial and temporal resolution of tumor cell behavior is required. The addition of GFP significantly enhances the versatility of this model, supporting applications in ovarian cancer research, therapeutic evaluation, and studies of tumor cell dynamics.
Organismo	Human
Tejido	Ovary
Enfermedad	Ovarian endometrioid adenocarcinoma
Lugar de metástasis	Primary tumor site (ovary)
Aplicaciones	Ovarian cancer imaging; GFP-based tumor tracking; in vivo fluorescence imaging; combination with parental A2780 for comparative studies; drug efficacy visualization

Edad	Age unspecified
Género	Female
Origen étnico	African American
Morfología	Epithelial-like
Tipo de célula	Epithelial cells
Propiedades de crecimiento	Adherent

Referencia	A2780-GFP (Cytion catalog number 305676)
Nivel de bioseguridad	1
NCBI_TaxID	9606
N.º de acceso de Cellosaurus	Not assigned (A2780-GFP reporter derivative; parental A2780 CVCL_1099)
Estado de los OGM	GMO-S1: This human A2780 ovarian cancer line contains a lentiviral GFP expression construct for fluorescence-based imaging. This classification applies only within Germany and may differ elsewhere.

Expresión de antígenos	GFP

Medio de cultivo	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Suplementos	Supplement the medium with 10% FBS
Reactivo de disociación	Accutase
Relación de división	1 to 5
Densidad de siembra	1 to 3 × 10⁴ cells/cm²
Renovación de fluidos	2 to 3 times per week
Medio de congelación	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Descongelación y cultivo de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Atmósfera de incubación	37°C, 5% CO₂, humidified atmosphere.
Condiciones de envío	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condiciones de almacenamiento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Número de lote	Tipo de certificado	Fecha	Número de catálogo
305676-040326	Certificado de análisis	08. Apr. 2026	305676

A2780-GFP

Información general

Características

Datos normativos

Datos biomoleculares

Manejo

Control de calidad y análisis molecular

Certificado de análisis (CoA)