U2OS-CRISPR-NUP96-Halo clone no.252 Cells
$540.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | This clonal stable cell line was generated by CRISPR-Cas9D10A nickase-assisted genome editing. |
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Organism | Human |
Tissue | Bone |
Disease | Osteosarcoma |
Characteristics
Age | 15 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | U-2 OS-CRISPR-NUP96-Halo clone no.252 (Cytion catalog number 300448) |
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Biosafety level | 1 |
Depositor | Dr. J. Ellenberg, EMBL Heidelberg |
Expression / Mutation
Protein expression | NUP96-Halo (endogenous nuclear pore complex protein 96, Halo tagged) |
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Handling
Culture Medium | McCoys 5a, w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion article number 820200a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 13,14
D13S317: 13,13
D16S539: 9,11
D5S818: 11,12
D7S820: 11,12
TH01: 9.3,9.3
TPOX: 11,12
vWA: 14,18
D3S1358: 16,16
D21S11: 31,32
D18S51: 12,14
Penta E: 10,13
Penta D: 9,9
D8S1179: 12,14
FGA: 20,20
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HLA alleles |
A*: 02:01:01, 32:01:01
B*: 44:02:01, 44:27:01
C*: 05:01:01, 07:04:01
DRB1*: 09:01:02G, 14:54:01
DQA1*: 01:04:01, 03:02:01
DQB1*: 03:03:02, 05:03:01
DPB1*: 02:01:02, 04:01:01
E: 01:01:01
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