SW-982 Cells
General information
Description | This cell line was established by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas. The histopathology evaluation pointed to an undifferentiated malignant tumor consistent with liposarcoma. |
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Organism | Human |
Tissue | Synovial |
Disease | Biphasic synovial sarcoma |
Synonyms | SW982, SW 982 |
Characteristics
Age | 25 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Mixed |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SW-982 (Cytion catalog number 300404) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 1-2, ES-D, 1, AK-1, 1, GLO-1, 1, Phenotype Frequency Product: 0.0192 |
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Karyotype | Hyperdiploid. Modal number = 48, range = 42 to 58. The rate of higher ploidies was 1.6%.Nine markers were common to all cells. These were: t(1q4q), del(5)(q31,q33), der(9)t(4,9)(q11,p24), t(8q12p), t(9q13q) and four others. Double minutes (DM) were seen in some cells (usually only one copy). Normal N9 was absent, N4, N8, and N13 were consistently single-copied and the x was double-copied. |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11,12
D13S317: 8,12,13
D16S539: 11,12
D5S818: 11,13
D7S820: 9,11
TH01: 9.3
TPOX: 9,11
vWA: 19,20
D3S1358: 15
D21S11: 28,30
D18S51: 16,18
Penta E: 13,15
Penta D: 10,13
D8S1179: 11,14
FGA: 21,24
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