SW-948 Cells
General information
Description | The cells are positive for keratin by immunoperoxidase staining. |
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Organism | Human |
Tissue | Colon |
Disease | Adenocarcinoma, grade III, Dukes' type C |
Synonyms | SW948, SW 948 |
Characteristics
Age | 81 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SW-948 (Cytion catalog number 300347) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | blood type O, Rh+ |
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Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 1-2, 6PGD, A, PEP-D, 1, ES-D, 1 |
Oncogenes | The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb and fos oncogenes. N-myc and sis expression were not detected. |
Tumorigenic | Yes, in nude mice |
Reverse transcriptase | Negative |
Products | Carcinoembryonic antigen (CEA) 7 ng/106 cells/10 days, colon specific antigen (CSAp) 750 units in 0.5 ml cell sonicate, keratin |
Mutational profile | SW-948 cells carry a heterozygous Kras mutation in codon 61: CAA(Wt Gln) >CTA(Leu) |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:15 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 1 to 2 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 10,11
D16S539: 11,12
D5S818: 11
D7S820: 9,11
TH01: 6,9.3
TPOX: 8,11
vWA: 16,18
D3S1358: 16,17
D21S11: 25.2,29
D18S51: 19
Penta E: 13
Penta D: 12
D8S1179: 12,14
FGA: 24
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HLA alleles |
A*: 01:01:01
B*: 08:01:01, 58:01:01
C*: 07:01:01, 07:18:01
DRB1*: 04:04:01, 13:02:01
DQA1*: 01:02:01, 03:01:01
DQB1*: 03:02:01, 06:04:01
DPB1*: 04:01:01
E: 01:01:01
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