SW-1353 Cells
General information
Description | This cell line was established in 1977 by A. Leibovitz. The initial culture medium was Leibovitz medium (L-15) containing cortisone and insulin plus 10% fetal bovine serum and antibiotics. |
---|---|
Organism | Human |
Tissue | Bone, right humerus |
Disease | Chondrosarcoma (Grade II) |
Synonyms | SW1353, SW 1353 |
Characteristics
Age | 72 years |
---|---|
Gender | Female |
Ethnicity | Caucasian |
Morphology | Fibroblast-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | SW-1353 (Cytion catalog number 300440) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Antigen expression | Antigen Expression: Blood type O- |
---|---|
Isoenzymes | G6PD, B, PGM1, 1, PGM3, 2, ES-D, 2, AK-1, 1, GLO-1, 2, Phenotype Frequency Product: 0.00009 |
Karyotype | hyperdiploid 47,xx, +7, Except for the trisomic N7 no other chromosome markers are evident |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
CSF1PO: 12
D13S317: 12,13
D16S539: 11,12
D5S818: 10,11
D7S820: 9,11
TH01: 6,9
TPOX: 8,11
vWA: 16,17
D3S1358: 15,18
D21S11: 30,32.2
D18S51: 13,17
Penta E: 12,14
Penta D: 10,11
D8S1179: 10,11
FGA: 22,23
|
HLA alleles |
A*: 24:02:01, 29:02:01
B*: 44:02:01, 44:03:01
C*: 02:02:02, 16:01:01
DRB1*: 07:01:01, 13:01:01
DQA1*: 01:03:01, 02:01:01
DQB1*: 02:02:01, 06:03:01
DPB1*: 02:01:02, 04:01:01
E: 01:01:01, 01:03
|