SW-13 Cells
General information
Description | Electron microscopic studies show many bulb gap junctions (BGJ). |
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Organism | Human |
Tissue | Adrenal gland |
Disease | Small cell carcinoma |
Synonyms | SW13, SW 13, Scott and White No. 13 |
Characteristics
Age | 55 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | SW-13 (Cytion catalog number 300349) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | G6PD, B |
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Virus susceptibility | Vesicular stomatitis (Indiana), poliovirus 1 |
Reverse transcriptase | Negative |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:8 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 11,12
D7S820: 8,10
TH01: 7,8
TPOX: 8
vWA: 17,19
D3S1358: 16
D21S11: 31,32.2
D18S51: 17
Penta E: 7,15
Penta D: 10,13
D8S1179: 10
FGA: 20
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