SW-1116 Cells
General information
Description | The cell line was isolated from colorectal adenocarcinoma tissue by Leibovitz et al. in 1976. They are positive for keratin by immunoperoxidase staining, but negative for CSAp (CSAp-) and colon antigen 3. The oncogenes c-myc, K-ras, H-ras, myb, sis and fos oncogenes are expressed, N-myc and N-ras expression was not observed. The tumor specific nuclear matrix proteins CC-4, CC-5 and CC-6 are expressed. |
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Organism | Human |
Tissue | Colon |
Disease | Adenocarcinoma |
Synonyms | SW1116, SW 1116 |
Characteristics
Age | 73 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SW-1116 (Cytion catalog number 300348) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | CEA positive |
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Isoenzymes | G6PD, B, PGM1, 1, PGM3, 1-2, 6PGD, A, ES-D, 1, PEP-D, 1 |
Oncogenes | myc +, myb + , ras +, fos +, sis +, p53 +, abl -, ros -, src - |
Tumorigenic | Yes, in nude mice |
Reverse transcriptase | Negative |
Products | Carcinoembryonic antigen (CEA) 2654 ng/106 cells/10 days, keratin |
Mutational profile | SW-1116 cells carry a mutation in codon 12 of Kras gene: GGT(Wt Gly) >GCT(Ala) |
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Fluid renewal | 1 to 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 10,11
D13S317: 11,14
D16S539: 9,12
D5S818: 11,12
D7S820: 12
TH01: 6
TPOX: 8,11
vWA: 14,19
D3S1358: 16
D21S11: 28,29
D18S51: 13
Penta E: 10,12
Penta D: 11,13
D8S1179: 10,11
FGA: 21,22
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HLA alleles |
A*: 23:01:01
B*: 44:03:01
C*: 04:01:01
DRB1*: 11:01:01
DQA1*: 05:05:01
DQB1*: 03:01:01
DPB1*: 04:02:01
E: 01:01:01
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