SNU-398 Cells

USD$550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Product number: 305274

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The SNU-398 cell line is derived from a hepatocellular carcinoma (HCC) of a human adult. This cell line is extensively used in liver cancer research to study the molecular mechanisms underlying hepatocarcinogenesis, tumor progression, and the development of therapeutic strategies. Hepatocellular carcinoma is a prevalent and deadly form of liver cancer, and SNU-398 cells provide a relevant model for investigating the genetic and epigenetic changes associated with this disease. SNU-398 cells exhibit an epithelial morphology and express markers characteristic of liver cancer, such as alpha-fetoprotein (AFP) and cytokeratins. They harbor genetic mutations and alterations typical of HCC, including mutations in the TP53 gene, which is commonly associated with many cancers. Researchers utilize SNU-398 cells to explore various signaling pathways involved in liver cancer, such as the Wnt/β-catenin, PI3K/Akt, and MAPK pathways. These cells are also employed in drug screening assays to evaluate the efficacy of chemotherapeutic agents and targeted therapies, as well as in studies investigating resistance mechanisms to conventional treatments. The SNU-398 cell line's importance in hepatocellular carcinoma research lies in its ability to model liver cancer biology and to contribute to the development of more effective therapies for liver cancer patients.
Organism	Human
Tissue	Liver
Disease	Adult hepatocellular carcinoma
Synonyms	SNU398, NCI-SNU-398

Age	42 years
Gender	Male
Ethnicity	Korean
Morphology	Epithelial
Growth properties	Mixed: adherent and floating clusters

Citation	SNU-398 (Cytion catalog number 305274)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0077

Surface antigens	Blood Type 0, Rh +
Viruses	Transformant: Hepatitis B virus (HBV)
Mutational profile	Mutation: CTNNB1, p.Ser37Cys (c.110C>G), heterozygous; Mutation: TP53, p.Ser215Ile (c.644G>T), heterozygous

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% heat-inactivated FBS, 25 mM HEPES
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305274-280824	Certificate of Analysis	26. May. 2025	305274

SNU-398 Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)

Organism	Human
Tissue	Stomach
Disease	Gastric signet ring cell adenocarcinoma