RD Cells
General information
Description | This line has recently been shown to be at least parental, if not identical, to TE-671. |
---|---|
Organism | Human |
Tissue | Embryonic |
Disease | Rhabdomyosarcoma |
Synonyms | R D, RD-2, RD 2, 130T, 130-T, 130 T, TE-32, TE 32, TE32, TE 32.T, Te 32.T |
Characteristics
Age | Embryo |
---|---|
Gender | Female |
Ethnicity | Caucasian |
Cell type | Spindle cells and large multinucleated cells |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | RD (Cytion catalog number 300401) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Isoenzymes | G6PD, B |
---|---|
Virus susceptibility | Poliovirus 1, vesicular stomatitis (Indiana), herpes simplex, vaccinia |
Reverse transcriptase | Negative |
Products | Myoglobin, myosin ATPase |
Karyotype | 2n=48 |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 is recommended |
Fluid renewal | Every 3 to 4 days |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 10,11
D13S317: 13
D16S539: 10,11
D5S818: 11
D7S820: 8,12
TH01: 9.3
TPOX: 9
vWA: 18
D3S1358: 15,17
D21S11: 28,29
D18S51: 13,18
Penta E: 12
Penta D: 11,13
D8S1179: 11,15
FGA: 20,21
|
HLA alleles |
A*: 01:01:01
B*: 37:01:01
C*: 06:02:01
DRB1*: 03:01:01
DQA1*: 05:01:01
DQB1*: 02:01:01
DPB1*: 01:01:01
E: 01:01:01
|