OVCAR3 Cells
Key points about OVCAR3 cells
Description | OVCAR-3 cells are a human ovarian cancer cell line established from the malignant ascites of a 60-year-old Caucasian female patient with progressive adenocarcinoma of the ovary, refractory to treatment with cyclophosphamide, adriamycin, and cisplatin. Ovcar 3 cells are used in a wide range of studies including drug resistance, particularly those involving DNA damage response biomarkers, homologous recombination repair, and the overall cell cycle dynamics, cancer cell biology, and gene expression studies. OVCAR-3 cells are epithelial in morphology and have been characterized by their high in vitro growth potential and their ability to form tumors in immunodeficient mice. These cells express several markers characteristic of ovarian carcinoma and have been utilized extensively to study the biology of ovarian cancer. OVCAR-3 cells are known to have a complex karyotype, with numerous chromosomal abnormalities that are typical of high-grade serous ovarian carcinomas. They are estrogen receptor-positive, which is relatively rare among ovarian cancer cell lines, and this feature is exploited in studies focusing on hormonal influences on ovarian cancer progression and treatment. In summary, the OVCAR3 cell line stands as a cornerstone in ovarian cancer research, offering a robust model for studying the complex interplay between hormonal influences, drug resistance, and the genetic underpinnings of high-grade ovarian serous adenocarcinoma. |
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Organism | Human |
Tissue | Ovary |
Disease | High grade ovarian serous adenocarcinoma |
Metastatic site | Ascites |
Synonyms | OVCAR-3, Ovcar-3, OVCAR.3, NIH:Ovcar-3, NIH:OVCAR3, NIH-OVCAR-3, NIHOVCAR3, OVCAR3, Ovcar3 |
Aspects of the ovarian cancer cell line NIH:OVCAR-3
Age | 60 years |
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Gender | Female |
Ethnicity | Caucasian |
Growth properties | Adherent |
Documentation
Citation | OVCAR3 (Cytion catalog number 300307) |
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Biosafety level | 1 |
Genetic profile
Receptors expressed | Androgen, estrogen, progesterone |
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Isoenzymes | G6PD, B, PGM1, 1, PGM3, 1, ES-D, 1, AK-1, 1, GLO-1, 1 |
Tumorigenic | Yes, in nude mice |
Ploidy status | Aneuploid |
Ovarian carcinoma cell line OVCAR3 culturing methods
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 40 to 60 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:6 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control of NIH:OVCAR-3 cells
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 11,12
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10
TH01: 9,9.3
TPOX: 8
vWA: 17
D3S1358: 17,18
D21S11: 29,31.2
D18S51: 13
Penta E: 7,13
Penta D: 12,13
D8S1179: 10,15
FGA: 21
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HLA alleles |
A*: 02:01:01, 29:02:01
B*: 07:02:01, 58:01:01
C*: 07:02:01, 07:18:01
DRB1*: 08:01:01, 08:04:01
DQA1*: 04:01:01, 04:01:02
DQB1*: 04:02:01
DPB1*: 02:01:02, 04:01:01
E: 01:01:01
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