MEG-01 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300482

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The MEG-01 cell line is a human megakaryoblast cell line established from the bone marrow of a 55-year-old male patient who was in the megakaryoblastic crisis phase of Chronic Myelogenous Leukemia (CML). This cell line was developed in 1983 at Nagoya University School of Medicine, Japan. The patient from whom MEG-01 was derived was positive for the Philadelphia chromosome (Ph1), a hallmark of CML. The MEG-01 cells exhibit a hyperdiploid karyotype with a modal chromosome number of 56 to 58, consistently showing the presence of the Ph1 chromosome, which is a result of the t(9;22) chromosomal translocation. MEG-01 cells have mixed growth properties, demonstrating both adherent and suspension characteristics in culture. These cells express several markers and antigens characteristic of megakaryocytic lineage, including CD41, CD61, and CDw14. They also test positive for cytoplasmic Factor VIII, surface GPIIb/IIIa, and various enzymatic activities such as periodic acid-Schiff (PAS) reaction, alpha naphthyl acetate esterase, and acid phosphatase. Interestingly, MEG-01 cells are negative for myeloperoxidase, alpha naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase, and alkaline phosphatase, which helps distinguish them from other myeloid cells. MEG-01 has been a valuable model for studying human megakaryopoiesis, platelet production, and the biosynthesis of proteins unique to the megakaryocytic lineage, such as platelet-derived growth factor (PDGF) and glycoproteins like GPIIb/IIIa. Due to its well-characterized genetic background and its ability to express key megakaryocyte markers, MEG-01 serves as a significant tool in the investigation of leukemia and platelet biogenesis mechanisms, although it is not intended for therapeutic or in vivo applications.
Organism	Human
Tissue	Bone marrow
Disease	Chronic myeloid leukemia
Synonyms	Meg-01, MEG01, Meg01

Age	55 years
Gender	Male
Ethnicity	East Asian
Morphology	Myoblast-like
Cell type	Megakaryoblast
Growth properties	Adherent/suspension

Citation	MEG-01 (Cytion catalog number 300482)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0425

Antigen expression	CD41 +, CD61 +, CDw14 +

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300482-160525	Certificate of Analysis	21. Jul. 2025	300482
300482-080824	Certificate of Analysis	23. May. 2025	300482

MEG-01 Cells

General information

Characteristics

Regulatory Data

Biomolecular Data

Handling

Quality Control & Molecular Analysis

Certificate of Analysis (CoA)