KHOS-312H Cells
General information
Description | The growth properties of KHOS-312H are similar to HOS (TE-85). The KHOS-312H does not represent a rescuable Kirsten murine sarcoma virus genome. |
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Organism | Human |
Tissue | Bone |
Disease | Osteosarcoma |
Synonyms | KHOS-321H, KHOS312H, KHOS321H |
Characteristics
Age | 13 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Fibroblast-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | KHOS-312H (Cytion catalog number 300447) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | No |
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Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
TH01: 6
TPOX: 8,11
vWA: 18
D3S1358: 15
D21S11: 31.2,32.2
D18S51: 14,17
Penta E: 7,12
Penta D: 9,10
D8S1179: 11,14
FGA: 24
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HLA alleles |
A*: 02:11:01
B*: 52:01:01
C*: 12:02:02
DRB1*: 15:02:01G, 16:02:01G
DQA1*: 01:02:02, 01:03:01
DQB1*: 05:02:01, 05:03:01
DPB1*: 02:01:02
E: 01:01:01
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