JEG-3 Cells
General information
Description | This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates. The cells are able to transform steroid precursors to estrone and estradiol. |
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Organism | Human |
Tissue | Placenta |
Disease | Choriocarcinoma |
Metastatic site | Brain |
Applications | Transfection host |
Synonyms | Jeg-3, JEG3, Jeg3, jeg3 |
Characteristics
Age | Fetus |
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Gender | Male |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | JEG-3 (Cytion catalog number 300222) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | PGM3, 1-2, PGM1, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, type B |
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Tumorigenic | Forms malignant tumor consistent with choriocarcinoma |
Products | hCG, human chorionic somatomammotrophin (placental lactogen), progesterone. |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 36 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:6 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 will result in a confluent monolayer within 2 to 3 days. |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Allow the cells to recover from the freezing process for 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 13,14
D5S818: 10,11
D7S820: 10,12
TH01: 9,9.3
TPOX: 8
vWA: 16
D3S1358: 15,16
D21S11: 30
D18S51: 14
Penta E: 8,12
Penta D: 9,12
D8S1179: 12
FGA: 23,24
D1S1656: 14,16
D6S1043: 11
D2S1338: 24
D12S391: 17,24
D19S433: 13,15
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HLA alleles |
A*: 01:01:01, 11:01:01
B*: 08:13, 35:01
C*: 04:01:01, 07:01:01
DRB1*: 01:03:01, 03:01:01
DQA1*: 01:01:01, 05:01:01
DQB1*: 02:01:01, 05:01:01
DPB1*: 01:01:01, 04:01:01
E: 01:01:01
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