ImWilms10T Cells
$600.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | The imWilms10T cell line was immortalized from Wilms10 cells, a primary tumor cell line which was established in 2015 by the group of Dr. Brigitte Royer-Pokora from tissue of a 2-year-old female patient with triphasic stromal predominant Wilms tumor. Immortalization using the catalytic subunit of human telomerase 8hTERT) in conjunction with the mutant (U19dl89-97tsA58) SV40 large T antigen (LT) resulted in the cytogenetically stable cell line imWilms10. |
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Organism | Human |
Tissue | Kidney |
Disease | Wilms Tumor |
Synonyms | ImWilms10 T, IM-WT-10 |
Characteristics
Age | 2 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Spindle-shaped |
Cell type | Wilms cells |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | ImWilms10T (Cytion catalog number 300419) |
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Biosafety level | 1 |
Depositor | B. Royer-Pokora |
Expression / Mutation
Mutational profile | WT1 mutation status: homozygous del WT1 within del11p13, LOH: no in 11p13 but UPD in 11p15, CTNNB1 mutation status: homozygous del TCT, p.DS45, UPD 3p |
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Handling
Culture Medium | MSCGM kit (from Lonza) |
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Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Fluid renewal | 1 to 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11,12
D13S317: 12,12
D16S539: 9,10
D5S818: 10,12
D7S820: 11,12
TH01: 8,6
TPOX: 8,11
vWA: 15,18
D3S1358: 17,17
D21S11: 29,30
D18S51: 14,16
Penta E: 7,10
Penta D: 10,13
D8S1179: 10,15
FGA: 22,24
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HLA alleles |
A*: 01:01:01, 11:01:01
B*: 18:01:01, 27:05:02
C*: 01:02:01, 12:03:01
DRB1*: 01:01:01, 11:04:01
DQA1*: 01:01:01, 05:05:01
DQB1*: 03:01:01, 05:01:01
DPB1*: 04:01:01G, 04:02:01G
E: 01:01:01
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