HeLa S3 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300384

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The HeLa S3 cell line is a clonal derivative of the original HeLa cell line, which was established from the cervical cancer cells of an adult woman. HeLa S3 cells are notable for their robust growth in suspension cultures and are frequently used in scientific research due to their adaptability to various medium formulations. This variant retains the key characteristics of the HeLa lineage, such as a rapid doubling time and a karyotype that is highly aneuploid, displaying numerous chromosomal abnormalities which are a hallmark of HeLa cells. HeLa S3 cells are widely utilized in virology, toxicology, and cancer research, particularly because they maintain the ability to be infected by poliovirus and other viruses, making them invaluable in pathogen-host interaction studies. They are also employed in the study of gene expression and regulation mechanisms under physiological and pathological conditions. The genetic and metabolic profiles of HeLa S3 have been extensively characterized, facilitating their use in high-throughput genetic screens and molecular biology applications.
Organism	Human
Tissue	Cervix
Disease	Adenocarcinoma
Synonyms	HeLa s3, HeLa-S3, HELA-S3, HeLa/S3, HeLa.S3, HeLa S 3, HeLa S-3, HeLaS3, S3-HeLa, S3 HeLa

Age	30 years
Gender	Female
Ethnicity	African American
Morphology	Epithelial-like
Growth properties	Adherent

Citation	HeLa S3 (Cytion catalog number 300384)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0058

Isoenzymes	G6PD, A
Virus susceptibility	Poliovirus 1, 2, 3, vesicular stomatitis (Indiana), encephalomyocarditis, adenovirus 5
Reverse transcriptase	Negative
Products	Keratin

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	1 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300384-618	Certificate of Analysis	23. May. 2025	300384

HeLa S3 Cells

General information

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Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)