HNO258 Cells
$540.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Organism | Human |
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Tissue | Oral cavity |
Disease | Head and neck squamous cell carcinoma (HNSCC) |
Characteristics
Age | 62 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | HNO258 (Cytion catalog number 300146) |
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Biosafety level | 1 |
Depositor | C. Herold-Mende |
Expression / Mutation
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | An initial ratio of 1:3 is recommended according to the growth rate |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8
TH01: 7
TPOX: 9,11
vWA: 16,17
D3S1358: 16
D21S11: 29
D18S51: 19
Penta E: 12,14
Penta D: 13
D8S1179: 10,13
FGA: 24
D1S1656: 12,16.3
D6S1043: 11,12
D2S1338: 19,20
D12S391: 14
D19S433: 19,20
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HLA alleles |
A*: 01:01:01, 25:01:01
B*: 07:02:01, 18:01:01
C*: 07:02:01, 12:03:01
DRB1*: 14:54:01, 15:01:01
DQA1*: 01:02:01, 01:04:01
DQB1*: 05:03:01, 06:02:01
DPB1*: 02:01:02G, 04:02:01G
E: 01:01:01
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