HEL 92.1.7
General information
Description | These cells differentiate spontaneously into erythroblast-like cells. Macrophage-like differentiation can be induced with phorbol esters such as TPA (12-O- tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid). |
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Organism | Human |
Tissue | Bone marrow |
Disease | Erythroleukemia |
Synonyms | HEL92.1.7, HEL-92.1.7, HEL-92-1-7, HEL-92_1_7, HEL-92, HEL92 |
Characteristics
Age | 30 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | Erythroblast |
Growth properties | Adherent/suspension |
Identifiers / Biosafety / Citation
Citation | HEL 92.1.7 (Cytion catalog number 300462) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | HLA A3, Aw32, Bw35, Ia+ |
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Products | Hemoglobin, globin (G gamma, A gamma, epsilon, zeta and alpha chains), beta-2-microglobulin, glycophorin |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium. |
Split ratio | A ratio of 1:3 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 7
TH01: 7
TPOX: 11
vWA: 14,17
D3S1358: 15
D21S11: 29,30.2
D18S51: 12,16
Penta E: 13,18
Penta D: 11,13
D8S1179: 13,15
FGA: 22,23
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HLA alleles |
A*: 03:01:01, 32:01:01
B*: 35:01:01, 35:08:01
C*: 04:01:01
DRB1*: 07:01:01, 13:03:01
DQA1*: 02:01:01, 05:05:01
DQB1*: 02:02:01, 03:01:01
DPB1*: 02:01:02, 04:01:01
E: 01:01:01, 01:03:02
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