ECV-304 Cells
General information
Description | DNA profiling studies revealed that STR patterns of the endothelial line ECV-304 and the human bladder line T24 were very similar, suggesting that ECV-304 was a derivative of T24. Furthermore, karyotypes of the two lines show two shared-marker chromosomes. Combined, these results show that ECV-304 is indeed a derivative of T24, a line that was developed years earlier. It is important to emphasize that all stocks of ECV show similar properties. |
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Organism | Human |
Tissue | Bladder |
Disease | Carcinoma |
Synonyms | ECV 304, ECV304, ECV, E304, T24(ECV304) |
Characteristics
Age | 82 years |
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Gender | Female |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | ECV-304 (Cytion catalog number 300452) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | Medium 199, w: 2.7 mM stable Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820101a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 10
D7S820: 10,11
TH01: 6
TPOX: 8,11
vWA: 17
D3S1358: 16
D21S11: 29
D18S51: 16,18
D8S1179: 14
FGA: 17,22
D2S1338: 20,23
D12S391: 18
D19S433: 13,14
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