CESS Cells
General information
Organism | Human |
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Tissue | Peripheral blood |
Disease | Acute myeloid leukemia |
Applications | Establishment of human T hybridoma cell lines |
Synonyms | Cess |
Characteristics
Gender | Male |
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Ethnicity | European |
Morphology | Lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | CESS (Cytion catalog number 300262) |
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Biosafety level | 1 |
Expression / Mutation
Viruses | Transformed by EBV |
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Products | IL-2 after induction with TRF (T cell-replacing factor) |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Seeding density | 1 x 10^4 cells/cm^2 is recommended |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Allow the cells to recover from the freezing process for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
TH01: 7,9.3
TPOX: 8,9
vWA: 16,17
D3S1358: 15,18
D21S11: 27,30
D18S51: 12,16
Penta E: 7,13
Penta D: 10,12
D8S1179: 13,14
FGA: 21,22
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