AS-30D Cells
General information
Description | Established in vitro from the AS-30D tumor ascites. |
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Organism | Rat |
Tissue | Liver |
Disease | Hepatocellular carcinoma |
Synonyms | A-S-30D, AS30D |
Characteristics
Age | 16 months |
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Gender | Female |
Morphology | Round cells, loosely adherent, floating |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | AS-30D (Cytion catalog number 500116) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in Sprague-Dawley rats |
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Viruses | RAP-test: Negative. |
Karyotype | Hypodiploid rat karyotype with 12% tetraploidy, 38 (35-41). |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 26 hours |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Split ratio | A ratio of 1:4 to 1:5 is recommended |
Seeding density | A seeding density of 1 x 10^6 cells/ml is recommended. |
Fluid renewal | Every 3 to 5 days |
Freezing recovery | After thawing, allow the cells to recover from the freezing process for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Rat_D2Wox37: 150,152
Rat_D19Wox11: 228
Rat_D10Wox8: 266
Rat_D4Wox7: 153,157
Rat_D2Wox27: 211
Rat_D5Rat33: 122,124,128
Rat_D10Wox11: 156
Rat_D1Wox23: 210,214
Rat_D12Wox1: 410
Rat_D6Wox2: 104
Rat_D8Wox7: 182
Rat_D6Cebr1: 225
SRY: x,Y
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