ACHN Cells
General information
Description | Human interferon inhibits cell growth. |
---|---|
Organism | Human |
Tissue | Kidney |
Disease | Adenocarcinoma |
Characteristics
Age | 22 years |
---|---|
Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | ACHN (Cytion catalog number 300117) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Receptors expressed | CAIx- (carbonic anhydrase Ix) |
---|---|
Protein expression | p53 positive |
Isoenzymes | CAIx- |
Tumorigenic | Yes, in nude mice |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 30 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will result in a confluent monolayer within 4 days. |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 11
D13S317: 12
D16S539: 12,13
D5S818: 12
D7S820: 9,11
TH01: 8
TPOX: 8,11
vWA: 16,17
D3S1358: 17
D21S11: 30
D18S51: 16
D8S1179: 12
FGA: 22
D2S1338: 17
D19S433: 14,15
|
HLA alleles |
A*: 01.01.1900 02:01
B*: 02.01.1900 01:01
C*: 07:01:01
DRB1*: 16:01:01
DQA1*: 01:02:02
DQB1*: 05:02:01
DPB1*: 02:01:02
E: 01:03:05
|