A72 Cells
General information
Description | A72 cells are a canine fibrosarcoma cell line derived from a spontaneously occurring tumor in a dog. These cells are used primarily in veterinary oncology research to study the biology, behavior, and treatment responses of canine fibrosarcomas. Their relevance extends to comparative oncology studies, where insights gained from canine cancers can be applied to human cancer research due to the biological similarities between certain canine and human tumors. The A72 cell line exhibits an adherent, fibroblast-like morphology and is known for its aggressive growth in vitro. It has been utilized to investigate various aspects of cancer cell biology, including proliferation, metastasis, and tumor cell interactions with the extracellular matrix. These cells are particularly valuable for assessing the efficacy of chemotherapeutic agents and exploring new therapeutic strategies, including immunotherapy and targeted therapies. A72 cells also provide a useful model for studying the molecular pathways involved in tumor growth and progression, such as signaling through the PI3K/Akt, MAPK, and other related pathways. They are instrumental in understanding the genetic and molecular underpinnings of fibrosarcoma, which can help identify potential biomarkers for diagnosis and targets for treatment in both veterinary and human oncology. |
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Organism | Canine |
Tissue | Muscle |
Disease | Carcinoma |
Synonyms | A 72, A-72 |
Characteristics
Age | 8 years |
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Gender | Female |
Morphology | Fibroblast-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | A72 (Cytion catalog number 602398) |
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Biosafety level | 1 |
Expression / Mutation
Virus susceptibility | Canine coronaviruses, canine adenovirus I, II, canine herpes viruses, canine parainfluenzavirus, canine parvovirus canine distemper virus, minute virus of canines |
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Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 24 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 will result in a confluent monolayer within 3 days. |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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